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@ARTICLE{Mhrmann:299861,
author = {L. Möhrmann$^*$ and L. Rostock and M. Werner$^*$ and M.
Oleś$^*$ and J. Arnold$^*$ and N. Paramasivam$^*$ and K.
Jöhrens and L. Rupp and M. Schmitz and D. Richter and S.
Uhrig$^*$ and M. A. Fröhlich$^*$ and B. Hutter$^*$ and J.
Hüllein$^*$ and A. Jahn$^*$ and M. Arlt$^*$ and E. E.
Möhrmann$^*$ and D. Hanf$^*$ and L. Gieldon and S.
Kreutzfeldt$^*$ and C. E. Heilig$^*$ and M.-V. Teleanu$^*$
and D. B. Lipka and K. Beck and A. Baude-Müller$^*$ and A.
Mock and I. Jelas and D. T. Rieke and M. Wiesweg and C.
Brandts$^*$ and M. Boerries and A. L. Illert and A.
Desuki$^*$ and T. Kindler$^*$ and A. M. Krackhardt and C. B.
Westphalen and P. Christopoulos and L. Apostolidis and A.
Stenzinger and M. Allgäuer and O. Neumann and I. Kerle$^*$
and P. Horak$^*$ and C. Heining$^*$ and H. Grosch and E.
Schröck$^*$ and D. Hübschmann$^*$ and S. Fröhling$^*$ and
H. Glimm},
title = {{G}enomic landscape and molecularly informed therapy in
thymic carcinoma and other advanced thymic epithelial
tumors.},
journal = {Med},
volume = {6},
number = {6},
issn = {2666-6340},
address = {Amsterdam},
publisher = {Elsevier},
reportid = {DKFZ-2025-00591},
pages = {100612},
year = {2025},
note = {#LA:B340#LA:B280#LA:W015 / 2025 Jun 13;6(6):100612/
Computational Oncology Group (CO), Molecular Precision
Oncology Program (MPOP), German Cancer Research Center
(DKFZ), translational Functional Cancer Genomics, DKFZ
Heidelberg, Heidelberg, Germany, Innovation and Service
Unit, German Cancer Research Center (DKFZ), Heidelberg,
Germany},
abstract = {Thymic epithelial tumors (TETs) are rare malignancies with
limited treatment options and underexplored molecular
features.We examined the genomic landscape and therapeutic
outcomes in 81 patients with advanced TETs, including thymic
carcinomas (TCs), thymomas, and thymic neuroendocrine
neoplasms (TNENs), who were enrolled in the MASTER trial, a
prospective observational precision oncology trial.Using
whole-genome-sequencing and whole-exome-sequencing analysis,
transcriptome analysis, and methylome analysis, we
identified distinct molecular features across TET subtypes,
including a higher tumor mutational burden in TC and
pathogenic germline variants in $18\%$ of cases. We
performed transcriptome- and methylome-based unsupervised
clustering and were able to divide TCs into immunologically
hot and cold subsets, with hot TCs exhibiting higher T cell
infiltration and significantly longer overall survival. In
65 out of 76 $(86\%)$ patients, we recommended molecularly
informed therapies, which were applied in 29 out of 65
$(45\%)$ cases, leading to a disease control rate of $62\%$
and an objective response rate of $23\%$ (both n = 26). The
progression-free survival ratio (PFSr) was > 1.3 in 8 out of
24 $(33\%)$ patients, 7 of them having TC. Among TCs,
patients achieved a mean PFSr of 1.4, indicating potential
therapeutic advantages in this subgroup. The PFSr between
the PFS of immune checkpoint inhibition and preceding
therapies was significantly higher in the hot cluster
compared to the cold cluster (median 1.7 vs. 0.3; p =
0.01945).Our findings expand the understanding of TET
biology and emphasize the role of precision oncology in
informing treatment decisions and improving outcomes for
patients with advanced TETs, particularly in TCs.},
keywords = {Translation to patients (Other) / molecular profiling
(Other) / multiomics (Other) / precision oncology (Other) /
prospective observational study (Other) / targeted therapy
(Other) / thymic carcinoma (Other) / thymic epithelial tumor
(Other) / thymic neuroendocrine neoplasm (Other) / thymoma
(Other) / whole-genome sequencing (Other)},
cin = {DD01 / B340 / FM01 / HD01 / B280 / W015},
ddc = {610},
cid = {I:(DE-He78)DD01-20160331 / I:(DE-He78)B340-20160331 /
I:(DE-He78)FM01-20160331 / I:(DE-He78)HD01-20160331 /
I:(DE-He78)B280-20160331 / I:(DE-He78)W015-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40107270},
doi = {10.1016/j.medj.2025.100612},
url = {https://inrepo02.dkfz.de/record/299861},
}