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@ARTICLE{Gewalt:301327,
      author       = {T. Gewalt and A. M. Dmitrieva and F. Elsner and X. Zhao and
                      D. D. Sieber and I. G. Kocak and Q. Yang and C. V. Orschel
                      and N. M. Eckert and B. Goebel and M. Nill and F. Peter and
                      A. Hartmann and F. Beleggia and M. Odenthal and H. C.
                      Reinhardt$^*$ and R. T. Ullrich and F. Graw and L. Meder},
      title        = {{TAT}-{CRE} inhalation enables tumor induction
                      corresponding to adenoviral {C}re-recombinase in a lung
                      cancer mouse model.},
      journal      = {Communications biology},
      volume       = {8},
      number       = {1},
      issn         = {2399-3642},
      address      = {London},
      publisher    = {Springer Nature},
      reportid     = {DKFZ-2025-00999},
      pages        = {741},
      year         = {2025},
      abstract     = {Cre-recombinase inducible model systems are extensively
                      used in cancer research to manipulate gene expression in
                      specific tissues and induce autochthonous tumor growth.
                      These systems often involve the cross-breeding of
                      genetically engineered organisms containing loxP-flanked
                      alleles with those expressing Cre-recombinase. This
                      approach, while effective, has the challenge of requiring
                      high numbers of animals due to breeding requirements. Other
                      frequently used tumor induction methods in cancer research
                      involve the direct application of viral Cre-recombinase
                      vectors. This approach presents the challenge of the
                      accessibility of facilities that meet the necessary safety
                      level. In this context, we perform a comprehensive
                      comparison between TAT-CRE (biosafety level S1) and
                      adenoviral Cre-recombinase induced (biosafety level S2) lung
                      adenocarcinomas driven by KrasG12D expression and Trp53
                      depletion. We use in vivo lung tumor monitoring via computed
                      tomography, single-cell RNA sequencing, immunohistochemistry
                      and flow cytometry to elucidate similarities and differences
                      between TAT-CRE and adenoviral Cre-recombinase induced lung
                      adenocarcinomas. TAT-CRE induced lung tumors present
                      differences in micro-vessels and macrophages but with
                      corresponding tumor onset and growth characteristics
                      compared to adenoviral-Cre recombinase induced lung tumors.
                      Taken together, TAT-CRE is a valuable genetic engineering
                      safety level S1 alternative for cancer induction and may be
                      implemented in other cancer models than lung cancer.},
      keywords     = {Animals / Integrases: genetics / Integrases: metabolism /
                      Integrases: administration $\&$ dosage / Lung Neoplasms:
                      genetics / Lung Neoplasms: pathology / Lung Neoplasms:
                      metabolism / Mice / Adenoviridae: genetics / Disease Models,
                      Animal / Adenocarcinoma of Lung: genetics / Adenocarcinoma
                      of Lung: pathology / Administration, Inhalation / Mice,
                      Inbred C57BL / Tumor Suppressor Protein p53: genetics / Cre
                      recombinase (NLM Chemicals) / Integrases (NLM Chemicals) /
                      Tumor Suppressor Protein p53 (NLM Chemicals)},
      cin          = {ED01},
      ddc          = {570},
      cid          = {I:(DE-He78)ED01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40360735},
      doi          = {10.1038/s42003-025-08146-0},
      url          = {https://inrepo02.dkfz.de/record/301327},
}