%0 Journal Article
%A Furkel, Jennifer
%A Zirkenbach, Vanessa A
%A Knoll, Maximilian
%A Öttl, Renate
%A Rein, Katrin
%A Abdollahi, Amir
%A Frey, Norbert
%A Konstandin, Mathias H
%A Kaya, Ziya
%T Cardiac Troponin I Antibodies Induce Cardiomyocyte Damage and Alter Cell Morphology.
%J International journal of molecular sciences
%V 26
%N 20
%@ 1422-0067
%C Basel
%I Molecular Diversity Preservation International
%M DKFZ-2025-02229
%P 10005
%D 2025
%Z #EA:E210#
%X Circulating heart-reactive autoantibodies (aAbs) detected in a variety of heart diseases (e.g., myocarditis, dilated cardiomyopathy, and myocardial infarction) have been associated with the progression of heart failure and a poor prognosis. However, the underlying mechanisms remain largely unknown. We investigated the effects of murine plasma containing aAbs against cardiac troponin I (cTnI) on neonatal rat cardiomyocytes (NRCMs). An autoimmune response to cTnI in A/J mice was induced, and anti-cTnI-aAbs were quantified. After 21 days, cardiac function, inflammation, fibrosis, and apoptosis were evaluated. In complementary in vitro liquid biopsy experiments, NRCMs were incubated with murine plasma containing high anti-cTnI-aAb levels or corresponding controls. Morphological phenotyping was performed using the C-MORE fluorescent image-based analysis workflow. Immunization with cTnI resulted in high anti-cTnI-aAb production, followed by myocardial inflammation, fibrosis, and impaired ejection fraction. NRCMs exposed to anti-cTnI-aAb-containing plasma showed reduced cell size, altered shape and radius, and elevated rate of dead cells in cell cycle analysis (p < 0.01, for 20
%K Animals
%K Myocytes, Cardiac: pathology
%K Myocytes, Cardiac: immunology
%K Myocytes, Cardiac: metabolism
%K Troponin I: immunology
%K Autoantibodies: immunology
%K Autoantibodies: blood
%K Rats
%K Mice
%K Fibrosis
%K Apoptosis
%K Animals, Newborn
%K Male
%K autoantibodies (Other)
%K cardiomyocytes (Other)
%K heart failure (Other)
%K morphology (Other)
%K troponin I (Other)
%K Troponin I (NLM Chemicals)
%K Autoantibodies (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41155300
%R 10.3390/ijms262010005
%U https://inrepo02.dkfz.de/record/305559