TY  - JOUR
AU  - Furkel, Jennifer
AU  - Zirkenbach, Vanessa A
AU  - Knoll, Maximilian
AU  - Öttl, Renate
AU  - Rein, Katrin
AU  - Abdollahi, Amir
AU  - Frey, Norbert
AU  - Konstandin, Mathias H
AU  - Kaya, Ziya
TI  - Cardiac Troponin I Antibodies Induce Cardiomyocyte Damage and Alter Cell Morphology.
JO  - International journal of molecular sciences
VL  - 26
IS  - 20
SN  - 1422-0067
CY  - Basel
PB  - Molecular Diversity Preservation International
M1  - DKFZ-2025-02229
SP  - 10005
PY  - 2025
N1  - #EA:E210#
AB  - Circulating heart-reactive autoantibodies (aAbs) detected in a variety of heart diseases (e.g., myocarditis, dilated cardiomyopathy, and myocardial infarction) have been associated with the progression of heart failure and a poor prognosis. However, the underlying mechanisms remain largely unknown. We investigated the effects of murine plasma containing aAbs against cardiac troponin I (cTnI) on neonatal rat cardiomyocytes (NRCMs). An autoimmune response to cTnI in A/J mice was induced, and anti-cTnI-aAbs were quantified. After 21 days, cardiac function, inflammation, fibrosis, and apoptosis were evaluated. In complementary in vitro liquid biopsy experiments, NRCMs were incubated with murine plasma containing high anti-cTnI-aAb levels or corresponding controls. Morphological phenotyping was performed using the C-MORE fluorescent image-based analysis workflow. Immunization with cTnI resulted in high anti-cTnI-aAb production, followed by myocardial inflammation, fibrosis, and impaired ejection fraction. NRCMs exposed to anti-cTnI-aAb-containing plasma showed reduced cell size, altered shape and radius, and elevated rate of dead cells in cell cycle analysis (p < 0.01, for 20
KW  - Animals
KW  - Myocytes, Cardiac: pathology
KW  - Myocytes, Cardiac: immunology
KW  - Myocytes, Cardiac: metabolism
KW  - Troponin I: immunology
KW  - Autoantibodies: immunology
KW  - Autoantibodies: blood
KW  - Rats
KW  - Mice
KW  - Fibrosis
KW  - Apoptosis
KW  - Animals, Newborn
KW  - Male
KW  - autoantibodies (Other)
KW  - cardiomyocytes (Other)
KW  - heart failure (Other)
KW  - morphology (Other)
KW  - troponin I (Other)
KW  - Troponin I (NLM Chemicals)
KW  - Autoantibodies (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:41155300
DO  - DOI:10.3390/ijms262010005
UR  - https://inrepo02.dkfz.de/record/305559
ER  -