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@ARTICLE{Schallenberg:305621,
author = {S. Schallenberg and G. Dernbach and S. Ruane and P.
Jurmeister and C. Böhm and K. Standvoss and S. Ghosh and M.
Frentsch and M.-P. Dragomir$^*$ and P. G. Keyl and C.
Friedrich and I.-K. Na$^*$ and S. Merkelbach-Bruse and A.
Quaas and N. Frost and K. Boschung and W. Randerath and G.
Schlachtenberger and M. Heldwein and U. Keilholz and K.
Hekmat and J.-C. Rückert and R. Büttner and A. Vasaturo
and D. Horst and L. Ruff and M. Alber and K.-R. Müller and
F. Klauschen$^*$},
title = {{AI}-powered spatial cell phenomics enhances risk
stratification in non-small cell lung cancer.},
journal = {Nature Communications},
volume = {16},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Springer Nature},
reportid = {DKFZ-2025-02269},
pages = {9701},
year = {2025},
abstract = {Risk stratification remains a critical challenge in
non-small cell lung cancer patients for optimal therapy
selection. In this study, we develop an artificial
intelligence-powered spatial cellomics approach that
combines histology, multiplex immunofluorescence imaging and
multimodal machine learning to characterize the complex
cellular relationships of 43 cell phenotypes in the tumor
microenvironment in a real-world retrospective cohort of
1168 non-small cell lung cancer patients from two large
German cancer centers. The model identifies cell niches
associated with survival and achieves a $14\%$ and $47\%$
improvement in risk stratification in the two main non-small
cell lung cancer subtypes, lung adenocarcinoma and squamous
cell carcinoma, respectively, combining niche patterns with
conventional cancer staging. Our results show that complex
immune cell niche patterns identify potentially undertreated
high-risk patients qualifying for adjuvant therapy. Our
approach highlights the potential of artificial intelligence
powered multiplex imaging analyses to better understand the
contribution of the tumor microenvironment to cancer
progression and to improve risk stratification and treatment
selection in non-small cell lung cancer.},
keywords = {Humans / Carcinoma, Non-Small-Cell Lung: pathology /
Carcinoma, Non-Small-Cell Lung: mortality / Carcinoma,
Non-Small-Cell Lung: diagnostic imaging / Lung Neoplasms:
pathology / Lung Neoplasms: mortality / Tumor
Microenvironment / Female / Male / Artificial Intelligence /
Retrospective Studies / Phenomics: methods / Middle Aged /
Aged / Risk Assessment: methods / Machine Learning /
Carcinoma, Squamous Cell: pathology},
cin = {BE01 / MU01},
ddc = {500},
cid = {I:(DE-He78)BE01-20160331 / I:(DE-He78)MU01-20160331},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:41184299},
doi = {10.1038/s41467-025-65783-z},
url = {https://inrepo02.dkfz.de/record/305621},
}