%0 Journal Article
%A Krickeberg, Naomi
%A Rammensee, Hans-Georg
%A Schilbach, Karin
%T Promiscuous class II-binding SARS-CoV-2-nuc derived vaccine-peptide induced extensive conventional, innate and unconventional T cell responses.
%J Frontiers in immunology
%V 16
%@ 1664-3224
%C Lausanne
%I Frontiers Media
%M DKFZ-2025-02644
%P 1676455
%D 2025
%X We describe the T-cell response of two healthy SARS-CoV-2-unexposed volunteers to a SARS-CoV-2 nucleoprotein-derived vaccine peptide predicted to promiscuously bind multiple HLA-DR allotypes. NGS-based bulk TCR-repertoire analysis of peptide-specific T-cell responses 4 (D2) and 27 (D1) weeks after vaccination identified CDR3 regions of TCRα, -β, -γ and -δ chains in T cells responding ex-vivo to the vaccine peptide LLLLDRLLNQLESKMS with IFNγ+-secretion. Adaptive repertoires were unique. Donors shared 15 TCRα and 9 TCRβ clonotypes, all public, showing no conserved motifs but TdT-independent 'neonatal' CDR3 regions close to the germline. Half the wtSARS-CoV-2 nucleocapsid-reactive adaptive clonotypes show preferential V-segment usage (6/64 Vα and 4-8/45 Vβ chains), and all share/show a N-nucleotide-encoded hydrophobicity in their CDR3 region. VδCα rearrangements (20.4
%K Humans
%K SARS-CoV-2: immunology
%K COVID-19: immunology
%K COVID-19: prevention & control
%K COVID-19 Vaccines: immunology
%K Immunity, Innate
%K T-Lymphocytes: immunology
%K Receptors, Antigen, T-Cell, alpha-beta: immunology
%K Nucleocapsid Proteins: immunology
%K Adult
%K HLA-DR Antigens: immunology
%K Vaccines, Subunit: immunology
%K CD1 restricted (Other)
%K SARS-CoV-2 (Other)
%K SARS-CoV-2 nucleoprotein peptide (Other)
%K T cell response (Other)
%K adaptive Vδ2γ9negative γδ T-cells (Other)
%K peptide vaccine (Other)
%K unconventional T cells (Other)
%K vaccine peptide reactive Vδ1 γδ T-cells (Other)
%K COVID-19 Vaccines (NLM Chemicals)
%K Receptors, Antigen, T-Cell, alpha-beta (NLM Chemicals)
%K Nucleocapsid Proteins (NLM Chemicals)
%K HLA-DR Antigens (NLM Chemicals)
%K Vaccines, Subunit (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41306962
%2 pmc:PMC12644032
%R 10.3389/fimmu.2025.1676455
%U https://inrepo02.dkfz.de/record/306607