TY  - JOUR
AU  - Krickeberg, Naomi
AU  - Rammensee, Hans-Georg
AU  - Schilbach, Karin
TI  - Promiscuous class II-binding SARS-CoV-2-nuc derived vaccine-peptide induced extensive conventional, innate and unconventional T cell responses.
JO  - Frontiers in immunology
VL  - 16
SN  - 1664-3224
CY  - Lausanne
PB  - Frontiers Media
M1  - DKFZ-2025-02644
SP  - 1676455
PY  - 2025
AB  - We describe the T-cell response of two healthy SARS-CoV-2-unexposed volunteers to a SARS-CoV-2 nucleoprotein-derived vaccine peptide predicted to promiscuously bind multiple HLA-DR allotypes. NGS-based bulk TCR-repertoire analysis of peptide-specific T-cell responses 4 (D2) and 27 (D1) weeks after vaccination identified CDR3 regions of TCRα, -β, -γ and -δ chains in T cells responding ex-vivo to the vaccine peptide LLLLDRLLNQLESKMS with IFNγ+-secretion. Adaptive repertoires were unique. Donors shared 15 TCRα and 9 TCRβ clonotypes, all public, showing no conserved motifs but TdT-independent 'neonatal' CDR3 regions close to the germline. Half the wtSARS-CoV-2 nucleocapsid-reactive adaptive clonotypes show preferential V-segment usage (6/64 Vα and 4-8/45 Vβ chains), and all share/show a N-nucleotide-encoded hydrophobicity in their CDR3 region. VδCα rearrangements (20.4
KW  - Humans
KW  - SARS-CoV-2: immunology
KW  - COVID-19: immunology
KW  - COVID-19: prevention & control
KW  - COVID-19 Vaccines: immunology
KW  - Immunity, Innate
KW  - T-Lymphocytes: immunology
KW  - Receptors, Antigen, T-Cell, alpha-beta: immunology
KW  - Nucleocapsid Proteins: immunology
KW  - Adult
KW  - HLA-DR Antigens: immunology
KW  - Vaccines, Subunit: immunology
KW  - CD1 restricted (Other)
KW  - SARS-CoV-2 (Other)
KW  - SARS-CoV-2 nucleoprotein peptide (Other)
KW  - T cell response (Other)
KW  - adaptive Vδ2γ9negative γδ T-cells (Other)
KW  - peptide vaccine (Other)
KW  - unconventional T cells (Other)
KW  - vaccine peptide reactive Vδ1 γδ T-cells (Other)
KW  - COVID-19 Vaccines (NLM Chemicals)
KW  - Receptors, Antigen, T-Cell, alpha-beta (NLM Chemicals)
KW  - Nucleocapsid Proteins (NLM Chemicals)
KW  - HLA-DR Antigens (NLM Chemicals)
KW  - Vaccines, Subunit (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:41306962
C2  - pmc:PMC12644032
DO  - DOI:10.3389/fimmu.2025.1676455
UR  - https://inrepo02.dkfz.de/record/306607
ER  -