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@ARTICLE{Krickeberg:306607,
      author       = {N. Krickeberg and H.-G. Rammensee$^*$ and K. Schilbach},
      title        = {{P}romiscuous class {II}-binding {SARS}-{C}o{V}-2-nuc
                      derived vaccine-peptide induced extensive conventional,
                      innate and unconventional {T} cell responses.},
      journal      = {Frontiers in immunology},
      volume       = {16},
      issn         = {1664-3224},
      address      = {Lausanne},
      publisher    = {Frontiers Media},
      reportid     = {DKFZ-2025-02644},
      pages        = {1676455},
      year         = {2025},
      abstract     = {We describe the T-cell response of two healthy
                      SARS-CoV-2-unexposed volunteers to a SARS-CoV-2
                      nucleoprotein-derived vaccine peptide predicted to
                      promiscuously bind multiple HLA-DR allotypes. NGS-based bulk
                      TCR-repertoire analysis of peptide-specific T-cell responses
                      4 (D2) and 27 (D1) weeks after vaccination identified CDR3
                      regions of TCRα, -β, -γ and -δ chains in T cells
                      responding ex-vivo to the vaccine peptide LLLLDRLLNQLESKMS
                      with IFNγ+-secretion. Adaptive repertoires were unique.
                      Donors shared 15 TCRα and 9 TCRβ clonotypes, all public,
                      showing no conserved motifs but TdT-independent 'neonatal'
                      CDR3 regions close to the germline. Half the wtSARS-CoV-2
                      nucleocapsid-reactive adaptive clonotypes show preferential
                      V-segment usage (6/64 Vα and 4-8/45 Vβ chains), and all
                      share/show a N-nucleotide-encoded hydrophobicity in their
                      CDR3 region. VδCα rearrangements $(20.4\%$ and $15.3\%$ of
                      the TCRα-repertoires, respectively), Vδ1Cδ
                      γδ-clonotypes homologous to public CD1-restricted Vδ1+
                      γδTCRs, and the induction of 'adaptive' Vδ2Vγ9negative T
                      cells support the role of innate T cells in the immune
                      response.},
      keywords     = {Humans / SARS-CoV-2: immunology / COVID-19: immunology /
                      COVID-19: prevention $\&$ control / COVID-19 Vaccines:
                      immunology / Immunity, Innate / T-Lymphocytes: immunology /
                      Receptors, Antigen, T-Cell, alpha-beta: immunology /
                      Nucleocapsid Proteins: immunology / Adult / HLA-DR Antigens:
                      immunology / Vaccines, Subunit: immunology / CD1 restricted
                      (Other) / SARS-CoV-2 (Other) / SARS-CoV-2 nucleoprotein
                      peptide (Other) / T cell response (Other) / adaptive
                      Vδ2γ9negative γδ T-cells (Other) / peptide vaccine
                      (Other) / unconventional T cells (Other) / vaccine peptide
                      reactive Vδ1 γδ T-cells (Other) / COVID-19 Vaccines (NLM
                      Chemicals) / Receptors, Antigen, T-Cell, alpha-beta (NLM
                      Chemicals) / Nucleocapsid Proteins (NLM Chemicals) / HLA-DR
                      Antigens (NLM Chemicals) / Vaccines, Subunit (NLM
                      Chemicals)},
      cin          = {TU01},
      ddc          = {610},
      cid          = {I:(DE-He78)TU01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41306962},
      pmc          = {pmc:PMC12644032},
      doi          = {10.3389/fimmu.2025.1676455},
      url          = {https://inrepo02.dkfz.de/record/306607},
}