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000306662 041__ $$aEnglish
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000306662 1001_ $$aKaschek, Lea$$b0
000306662 245__ $$aFura-10, unlike fura-2, is suitable for long-term calcium imaging in natural killer (NK) cells without compromising cytotoxicity and can be combined with target cell death analysis.
000306662 260__ $$aEdinburgh [u.a.]$$bChurchill Livingstone$$c2026
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000306662 500__ $$aVolume 133, January 2026, 103091
000306662 520__ $$aThere are compelling reasons to opt for primary human natural killer (NK) cells when validating Ca2+ indicators. 1.) NK cells exhibit a high degree of vulnerability to stressors such as indicator loading or light exposure. 2.) The lack of research on NK Ca2+ signaling underscores the necessity for developing reliable assays. 3.) The increased utilization of NK cell therapies necessitates a more profound comprehension of Ca2+ dependent signal transduction. Consequently, an assay was developed to monitor cytosolic Ca2+ signals in individual NK cells simultaneously with their cytotoxic function against cancer cells. We used this assay to assess the suitability of fura-2, fura-PE3, fura-8, fura-10 or fura-red for quantifying Ca2+ signals in NK cells without compromising their cytotoxic function. In contrast to the widely used fura-2, its red-shifted derivative fura-10 did not interfere with NK cytotoxicity over several hours. It exhibited a superior signal-to-noise ratio and good dynamic range, accompanied by minimal bleaching or leakage. Fura-8 and fura-red also preserved NK cell cytotoxicity, but had other disadvantages compared to fura-10. We successfully used fura-10 to report Ca2+ signals in NK cells from blood donors and patients diagnosed with lymphoma and leukemia over several hours at 37 °C during apoptotic or necrotic killing of different cancer cells (K562, THP1, OCI-AML2, and TMD8). Additionally, we show that fura-10 is well suited to report Ca2+ signals in intact murine pancreatic islets, another stress-sensitive cell preparation. Consequently, fura-10 is an optimal choice for measuring Ca²⁺ in primary human NK cells and other primary cell preparations.
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000306662 650_7 $$2Other$$aCalcium
000306662 650_7 $$2Other$$aCytotoxicity
000306662 650_7 $$2Other$$aFura-10
000306662 650_7 $$2Other$$aFura-2
000306662 650_7 $$2Other$$aNatural killer (NK) cell
000306662 650_7 $$2Other$$aPancreatic islets
000306662 650_7 $$2Other$$acancer
000306662 7001_ $$aVialle, Joanne$$b1
000306662 7001_ $$aStopper, Gebhard$$b2
000306662 7001_ $$aHoffmann, Markus D A$$b3
000306662 7001_ $$aZöphel, Sylvia$$b4
000306662 7001_ $$aJansky, Johanna$$b5
000306662 7001_ $$aKüchler, Nadja$$b6
000306662 7001_ $$aSchäfer, Gertrud$$b7
000306662 7001_ $$aMoter, Alina$$b8
000306662 7001_ $$0P:(DE-He78)c95d366c49b6ef22689f00d0832a237f$$aWendel, Philipp$$b9$$udkfz
000306662 7001_ $$aNeumann, Frank$$b10
000306662 7001_ $$aSchormann, Claudia$$b11
000306662 7001_ $$0P:(DE-HGF)0$$aUllrich, Evelyn$$b12
000306662 7001_ $$aRoma, Leticia Prates$$b13
000306662 7001_ $$aKummerow, Carsten$$b14
000306662 7001_ $$aThurner, Lorenz$$b15
000306662 7001_ $$aSchwarz, Eva C$$b16
000306662 7001_ $$aHoth, Markus$$b17
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