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@ARTICLE{Kaschek:306662,
      author       = {L. Kaschek and J. Vialle and G. Stopper and M. D. A.
                      Hoffmann and S. Zöphel and J. Jansky and N. Küchler and G.
                      Schäfer and A. Moter and P. Wendel$^*$ and F. Neumann and
                      C. Schormann and E. Ullrich$^*$ and L. P. Roma and C.
                      Kummerow and L. Thurner and E. C. Schwarz and M. Hoth},
      title        = {{F}ura-10, unlike fura-2, is suitable for long-term calcium
                      imaging in natural killer ({NK}) cells without compromising
                      cytotoxicity and can be combined with target cell death
                      analysis.},
      journal      = {Cell calcium},
      volume       = {133},
      issn         = {0143-4160},
      address      = {Edinburgh [u.a.]},
      publisher    = {Churchill Livingstone},
      reportid     = {DKFZ-2025-02655},
      pages        = {103091},
      year         = {2025},
      abstract     = {There are compelling reasons to opt for primary human
                      natural killer (NK) cells when validating Ca2+ indicators.
                      1.) NK cells exhibit a high degree of vulnerability to
                      stressors such as indicator loading or light exposure. 2.)
                      The lack of research on NK Ca2+ signaling underscores the
                      necessity for developing reliable assays. 3.) The increased
                      utilization of NK cell therapies necessitates a more
                      profound comprehension of Ca2+ dependent signal
                      transduction. Consequently, an assay was developed to
                      monitor cytosolic Ca2+ signals in individual NK cells
                      simultaneously with their cytotoxic function against cancer
                      cells. We used this assay to assess the suitability of
                      fura-2, fura-PE3, fura-8, fura-10 or fura-red for
                      quantifying Ca2+ signals in NK cells without compromising
                      their cytotoxic function. In contrast to the widely used
                      fura-2, its red-shifted derivative fura-10 did not interfere
                      with NK cytotoxicity over several hours. It exhibited a
                      superior signal-to-noise ratio and good dynamic range,
                      accompanied by minimal bleaching or leakage. Fura-8 and
                      fura-red also preserved NK cell cytotoxicity, but had other
                      disadvantages compared to fura-10. We successfully used
                      fura-10 to report Ca2+ signals in NK cells from blood donors
                      and patients diagnosed with lymphoma and leukemia over
                      several hours at 37 °C during apoptotic or necrotic killing
                      of different cancer cells (K562, THP1, OCI-AML2, and TMD8).
                      Additionally, we show that fura-10 is well suited to report
                      Ca2+ signals in intact murine pancreatic islets, another
                      stress-sensitive cell preparation. Consequently, fura-10 is
                      an optimal choice for measuring Ca²⁺ in primary human NK
                      cells and other primary cell preparations.},
      keywords     = {Calcium (Other) / Cytotoxicity (Other) / Fura-10 (Other) /
                      Fura-2 (Other) / Natural killer (NK) cell (Other) /
                      Pancreatic islets (Other) / cancer (Other)},
      cin          = {FM01},
      ddc          = {610},
      cid          = {I:(DE-He78)FM01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41314008},
      doi          = {10.1016/j.ceca.2025.103091},
      url          = {https://inrepo02.dkfz.de/record/306662},
}