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| 024 | 7 | _ | |a 10.1016/j.ceca.2025.103091 |2 doi |
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| 024 | 7 | _ | |a 0143-4160 |2 ISSN |
| 024 | 7 | _ | |a 1532-1991 |2 ISSN |
| 037 | _ | _ | |a DKFZ-2025-02655 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Kaschek, Lea |b 0 |
| 245 | _ | _ | |a Fura-10, unlike fura-2, is suitable for long-term calcium imaging in natural killer (NK) cells without compromising cytotoxicity and can be combined with target cell death analysis. |
| 260 | _ | _ | |a Edinburgh [u.a.] |c 2026 |b Churchill Livingstone |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1767965140_3737040 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
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| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 500 | _ | _ | |a Volume 133, January 2026, 103091 |
| 520 | _ | _ | |a There are compelling reasons to opt for primary human natural killer (NK) cells when validating Ca2+ indicators. 1.) NK cells exhibit a high degree of vulnerability to stressors such as indicator loading or light exposure. 2.) The lack of research on NK Ca2+ signaling underscores the necessity for developing reliable assays. 3.) The increased utilization of NK cell therapies necessitates a more profound comprehension of Ca2+ dependent signal transduction. Consequently, an assay was developed to monitor cytosolic Ca2+ signals in individual NK cells simultaneously with their cytotoxic function against cancer cells. We used this assay to assess the suitability of fura-2, fura-PE3, fura-8, fura-10 or fura-red for quantifying Ca2+ signals in NK cells without compromising their cytotoxic function. In contrast to the widely used fura-2, its red-shifted derivative fura-10 did not interfere with NK cytotoxicity over several hours. It exhibited a superior signal-to-noise ratio and good dynamic range, accompanied by minimal bleaching or leakage. Fura-8 and fura-red also preserved NK cell cytotoxicity, but had other disadvantages compared to fura-10. We successfully used fura-10 to report Ca2+ signals in NK cells from blood donors and patients diagnosed with lymphoma and leukemia over several hours at 37 °C during apoptotic or necrotic killing of different cancer cells (K562, THP1, OCI-AML2, and TMD8). Additionally, we show that fura-10 is well suited to report Ca2+ signals in intact murine pancreatic islets, another stress-sensitive cell preparation. Consequently, fura-10 is an optimal choice for measuring Ca²⁺ in primary human NK cells and other primary cell preparations. |
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| 650 | _ | 7 | |a Calcium |2 Other |
| 650 | _ | 7 | |a Cytotoxicity |2 Other |
| 650 | _ | 7 | |a Fura-10 |2 Other |
| 650 | _ | 7 | |a Fura-2 |2 Other |
| 650 | _ | 7 | |a Natural killer (NK) cell |2 Other |
| 650 | _ | 7 | |a Pancreatic islets |2 Other |
| 650 | _ | 7 | |a cancer |2 Other |
| 700 | 1 | _ | |a Vialle, Joanne |b 1 |
| 700 | 1 | _ | |a Stopper, Gebhard |b 2 |
| 700 | 1 | _ | |a Hoffmann, Markus D A |b 3 |
| 700 | 1 | _ | |a Zöphel, Sylvia |b 4 |
| 700 | 1 | _ | |a Jansky, Johanna |b 5 |
| 700 | 1 | _ | |a Küchler, Nadja |b 6 |
| 700 | 1 | _ | |a Schäfer, Gertrud |b 7 |
| 700 | 1 | _ | |a Moter, Alina |b 8 |
| 700 | 1 | _ | |a Wendel, Philipp |0 P:(DE-He78)c95d366c49b6ef22689f00d0832a237f |b 9 |u dkfz |
| 700 | 1 | _ | |a Neumann, Frank |b 10 |
| 700 | 1 | _ | |a Schormann, Claudia |b 11 |
| 700 | 1 | _ | |a Ullrich, Evelyn |0 P:(DE-HGF)0 |b 12 |
| 700 | 1 | _ | |a Roma, Leticia Prates |b 13 |
| 700 | 1 | _ | |a Kummerow, Carsten |b 14 |
| 700 | 1 | _ | |a Thurner, Lorenz |b 15 |
| 700 | 1 | _ | |a Schwarz, Eva C |b 16 |
| 700 | 1 | _ | |a Hoth, Markus |b 17 |
| 773 | _ | _ | |a 10.1016/j.ceca.2025.103091 |g Vol. 133, p. 103091 - |0 PERI:(DE-600)2002623-7 |p 103091 |t Cell calcium |v 133 |y 2026 |x 0143-4160 |
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