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@ARTICLE{Salmon:307458,
      author       = {C. Salmon and R. P. L. Neves and N. H. Stoecklein and S.-T.
                      Liffers$^*$ and J. Siveke$^*$ and J. D. Kuhlmann$^*$ and P.
                      Wimberger$^*$ and P. Buderath and R. Kimmig and S.
                      Kasimir-Bauer},
      title        = {{P}henotypic and genotypic characterization of single
                      circulating tumor cells in the follow-up of high-grade
                      serous ovarian cancer.},
      journal      = {Molecular oncology},
      volume       = {nn},
      issn         = {1574-7891},
      address      = {Hoboken, NJ},
      publisher    = {John Wiley $\&$ Sons, Inc.},
      reportid     = {DKFZ-2025-03057},
      pages        = {nn},
      year         = {2025},
      note         = {epub},
      abstract     = {Single circulating tumor cell (sCTC) analysis enables the
                      determination of predominant CTC phenotypes and genotypes.
                      We previously demonstrated the feasibility of sCTC detection
                      and genomic characterization in high-grade serous ovarian
                      cancer (HGSOC) by combining immune-magnetic enrichment and
                      image-based sorting, followed by whole-genome amplification
                      (WGA) and next-generation sequencing-based copy number
                      alteration analysis (CNA). Here we aimed to improve our
                      workflow by incorporating HGSOC-specific markers, folate
                      receptor alpha (FRα), and markers to identify epithelial
                      (cytokeratin) and mesenchymal (vimentin) phenotypes for the
                      phenotypic as well as genotypic analysis of sCTCs over the
                      course of treatment in 42 HGSOC patients. We detected a
                      significant reduction of FRα-positive cells (P = 0.0205)
                      and an expansion of cells with a high nuclear staining and
                      no target antigen expression (P = 0.002). Before treatment,
                      sCTCs showed an enrichment in CNAs of Chromosomes 2, 7, and
                      12, while CNA dynamics of sCTCs suggested a potential
                      selection of distinct CNAs specific to the homologous
                      recombination pathway. sCTCs revealed persistent CNAs in the
                      CDK4 and emerging ones in the ALK oncogene. Notably, primary
                      tumors revealed considerable fractions of shared genomic
                      aberrations.},
      keywords     = {genotype (Other) / high‐grade serous ovarian cancer
                      (Other) / phenotype (Other) / single cell sequencing (Other)
                      / single circulating tumor cells (Other)},
      cin          = {ED01 / DD01},
      ddc          = {610},
      cid          = {I:(DE-He78)ED01-20160331 / I:(DE-He78)DD01-20160331},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:41437733},
      doi          = {10.1002/1878-0261.70193},
      url          = {https://inrepo02.dkfz.de/record/307458},
}