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@ARTICLE{Scheck:307510,
author = {J. Scheck$^*$ and B. Boztepe$^*$ and J. M. Kernbach and K.
Karimian-Jazi and L. Heinz and K. Schregel and V. Sturm and
M. Schell and J. Bojcevski$^*$ and M. Fischer and R. Eurich
and I. Poschke$^*$ and J. Schwarz and D. A. Agardy$^*$ and
S. Jünger$^*$ and C. Schulz and F. Althammer and A. R.
Osidach and A. Abdollahi$^*$ and L. Bunse$^*$ and V.
Venkataramani$^*$ and S. M. Pfister$^*$ and F. Winkler$^*$
and T. Kessler$^*$ and W. Wick$^*$ and S. Heiland and M.
Platten$^*$ and C. Rodell and M. Bendszus and I.
Weidenfeld$^*$ and M. Breckwoldt$^*$},
title = {{M}ultimodality mapping of immunotherapy distribution as a
predictive marker in glioma.},
journal = {Neuro-Oncology},
volume = {nn},
issn = {1522-8517},
address = {Oxford},
publisher = {Oxford Univ. Press},
reportid = {DKFZ-2026-00015},
pages = {nn},
year = {2025},
note = {#EA:E210#LA:E210#LA:D170# / epub},
abstract = {Scarce T cell infiltration, immunosuppressive
tumor-associated macrophages and ineffective drug delivery
drive glioma progression and limit treatment efficacy.
Mapping immunotherapy distribution by multimodality imaging
might be a biomarker that could aid tumor monitoring and
guide therapy development.To assess drug delivery, we
developed a MRI-lightsheet microscopy platform (MR-LSM) to
monitor immunotherapy at the cellular level in two
immunocompetent glioma models (Gl261, SB28). The
atezolizumab (PD-L1 inhibitor) subgroup of the multicenter
N2M2/NOA20 trial in MGMT unmethylated GBM patients was
assessed by CNN analysis and correlated to progression free
survival.In contrast to the conventional Gl261 glioma model,
SB28 gliomas are characterized by poor immunogenicity and
resistance to Toll-like receptor (TLR) 7 targeted therapy
delivered by CDNP-R848 nanoparticles. SB28 resistance is
driven by microvascular pathology, vasogenic edema and drug
off-targeting to peritumoral edema and white matter tracts.
Vascular endothelial growth factor (VEGF) inhibition in
conjunction with irradiation and dual immunotherapy (DIR)
targeting innate (CDNP-R848) and adaptive immunity
(anti-CTLA-4) breaks resistance, increases survival and
reverses drug off-targeting. Mechanistically, tumor control
is orchestrated by vascular normalization, enhanced CD8+ T
cell influx and a proinflammatory shift of myeloid cells
along with strong IL-12/IL-13 upregulation. In a
translational analysis of the multicenter N2M2/NOA20 trial
we validate that edema and microvascular pathology are
associated with poor prognosis in glioblastoma patients
treated with checkpoint immunotherapy and that patients
without edema have increased PFS.We develop a customizable
imaging platform to map drug delivery to glioma with broad
applicability in neuroscience and neurooncology.},
keywords = {blood-brain barrier disruption (Other) / drug distribution
(Other) / glioma (Other) / immunotherapy (Other) / tumor
microenvironment (Other)},
cin = {E210 / D170 / HD01 / B062 / B320},
ddc = {610},
cid = {I:(DE-He78)E210-20160331 / I:(DE-He78)D170-20160331 /
I:(DE-He78)HD01-20160331 / I:(DE-He78)B062-20160331 /
I:(DE-He78)B320-20160331},
pnm = {312 - Funktionelle und strukturelle Genomforschung
(POF4-312)},
pid = {G:(DE-HGF)POF4-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:41476211},
doi = {10.1093/neuonc/noaf295},
url = {https://inrepo02.dkfz.de/record/307510},
}