Home > Institute Collections > W500 > Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. > print

001     305779
005     20251113160256.0
024 7 _ |a 10.1016/j.stem.2019.03.018
|2 doi
024 7 _ |a pmid:31031139
|2 pmid
024 7 _ |a pmc:PMC6561721
|2 pmc
024 7 _ |a 1934-5909
|2 ISSN
024 7 _ |a 1875-9777
|2 ISSN
037 _ _ |a DKFZ-2025-02396
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Benchetrit, Hana
|b 0
245 _ _ |a Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts.
260 _ _ |a Amsterdam [u.a.]
|c 2019
|b Elsevier
336 7 _ |a article
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336 7 _ |a Output Types/Journal article
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336 7 _ |a Journal Article
|b journal
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|0 PUB:(DE-HGF)16
|s 1763046146_4080000
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336 7 _ |a ARTICLE
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336 7 _ |a Journal Article
|0 0
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500 _ _ |a #DKFZ-MOST-Ca177#
520 _ _ |a Following fertilization, totipotent cells undergo asymmetric cell divisions, resulting in three distinct cell types in the late pre-implantation blastocyst: epiblast (Epi), primitive endoderm (PrE), and trophectoderm (TE). Here, we aim to understand whether these three cell types can be induced from fibroblasts by one combination of transcription factors. By utilizing a sophisticated fluorescent knockin reporter system, we identified a combination of five transcription factors, Gata3, Eomes, Tfap2c, Myc, and Esrrb, that can reprogram fibroblasts into induced pluripotent stem cells (iPSCs), induced trophoblast stem cells (iTSCs), and induced extraembryonic endoderm stem cells (iXENs), concomitantly. In-depth transcriptomic, chromatin, and epigenetic analyses provide insights into the molecular mechanisms that underlie the reprogramming process toward the three cell types. Mechanistically, we show that the interplay between Esrrb and Eomes during the reprogramming process determines cell fate, where high levels of Esrrb induce a XEN-like state that drives pluripotency and high levels of Eomes drive trophectodermal fate.
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
650 _ 7 |a Eomes
|2 Other
650 _ 7 |a Esrrb
|2 Other
650 _ 7 |a early embryonic development
|2 Other
650 _ 7 |a induced extraembryonic endoderm stem cells
|2 Other
650 _ 7 |a induced pluripotent stem cells
|2 Other
650 _ 7 |a induced trophoblast stem cells
|2 Other
650 _ 7 |a inner cell mass
|2 Other
650 _ 7 |a primitive endoderm
|2 Other
650 _ 7 |a reprogramming
|2 Other
650 _ 7 |a trophectoderm
|2 Other
650 _ 7 |a EOMES protein, human
|2 NLM Chemicals
650 _ 7 |a ESRRB protein, human
|2 NLM Chemicals
650 _ 7 |a Receptors, Estrogen
|2 NLM Chemicals
650 _ 7 |a T-Box Domain Proteins
|2 NLM Chemicals
650 _ 7 |a Transcription Factors
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Blastocyst: physiology
|2 MeSH
650 _ 2 |a Cell Differentiation
|2 MeSH
650 _ 2 |a Cell Lineage
|2 MeSH
650 _ 2 |a Cells, Cultured
|2 MeSH
650 _ 2 |a Cellular Reprogramming
|2 MeSH
650 _ 2 |a Embryo Implantation
|2 MeSH
650 _ 2 |a Endoderm: physiology
|2 MeSH
650 _ 2 |a Fibroblasts: physiology
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: physiology
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Receptors, Estrogen: genetics
|2 MeSH
650 _ 2 |a Receptors, Estrogen: metabolism
|2 MeSH
650 _ 2 |a T-Box Domain Proteins: genetics
|2 MeSH
650 _ 2 |a T-Box Domain Proteins: metabolism
|2 MeSH
650 _ 2 |a Transcription Factors: metabolism
|2 MeSH
650 _ 2 |a Trophoblasts: physiology
|2 MeSH
700 1 _ |a Jaber, Mohammad
|b 1
700 1 _ |a Zayat, Valery
|b 2
700 1 _ |a Sebban, Shulamit
|b 3
700 1 _ |a Pushett, Avital
|b 4
700 1 _ |a Makedonski, Kirill
|b 5
700 1 _ |a Zakheim, Zvi
|b 6
700 1 _ |a Radwan, Ahmed
|b 7
700 1 _ |a Maoz, Noam
|b 8
700 1 _ |a Lasry, Rachel
|b 9
700 1 _ |a Renous, Noa
|b 10
700 1 _ |a Inbar, Michal
|b 11
700 1 _ |a Ram, Oren
|b 12
700 1 _ |a Kaplan, Tommy
|b 13
700 1 _ |a Buganim, Yosef
|b 14
773 _ _ |a 10.1016/j.stem.2019.03.018
|g Vol. 24, no. 6, p. 983 - 994.e7
|0 PERI:(DE-600)2375356-0
|n 6
|p 983 - 994.e7
|t Cell stem cell
|v 24
|y 2019
|x 1934-5909
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