TY  - JOUR
AU  - Harari, Anna
AU  - Zoltsman, Guy
AU  - Levin, Tal
AU  - Rosenzweig, Rina
TI  - Hsp104 N-terminal domain interaction with substrates plays a regulatory role in protein disaggregation.
JO  - The FEBS journal
VL  - 289
IS  - 17
SN  - 0014-2956
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - DKFZ-2025-02413
SP  - 5359 - 5377
PY  - 2022
N1  - #DKFZ-MOST-Ca183#
AB  - Heat shock protein 104 (Hsp104) protein disaggregases are powerful molecular machines that harness the energy derived from ATP binding and hydrolysis to disaggregate a wide range of protein aggregates and amyloids, as well as to assist in yeast prion propagation. Little is known, however, about how Hsp104 chaperones recognize such a diversity of substrates, or indeed the contribution of the substrate-binding N-terminal domain (NTD) to Hsp104 function. Herein, we present a NMR spectroscopy study, which structurally characterizes the Hsp104 NTD-substrate interaction. We show that the NTD includes a substrate-binding groove that specifically recognizes exposed hydrophobic stretches in unfolded, misfolded, amyloid and prion substrates of Hsp104. In addition, we find that the NTD itself has chaperoning activities which help to protect the exposed hydrophobic regions of its substrates from further misfolding and aggregation, thereby priming them for threading through the Hsp104 central channel. We further demonstrate that mutations to this substrate-binding groove abolish Hsp104 activation by client proteins and keep the chaperone in a partially inhibited state. The Hsp104 variant with these mutations also exhibited significantly reduced disaggregation activity and cell survival at extreme temperatures. Together, our findings provide both a detailed characterization of the NTD-substrate complex and insight into the functional regulatory role of the NTD in protein disaggregation and yeast thermotolerance.
KW  - HSP70 Heat-Shock Proteins: metabolism
KW  - Heat-Shock Proteins: metabolism
KW  - Molecular Chaperones: metabolism
KW  - Prions: genetics
KW  - Saccharomyces cerevisiae: genetics
KW  - Saccharomyces cerevisiae: metabolism
KW  - Saccharomyces cerevisiae Proteins: metabolism
KW  - Hsp104 (Other)
KW  - NMR spectroscopy (Other)
KW  - molecular chaperones (Other)
KW  - protein disaggregation (Other)
KW  - HSP70 Heat-Shock Proteins (NLM Chemicals)
KW  - Heat-Shock Proteins (NLM Chemicals)
KW  - Molecular Chaperones (NLM Chemicals)
KW  - Prions (NLM Chemicals)
KW  - Saccharomyces cerevisiae Proteins (NLM Chemicals)
KW  - HsP104 protein, S cerevisiae (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:35305079
C2  - pmc:PMC9541529
DO  - DOI:10.1111/febs.16441
UR  - https://inrepo02.dkfz.de/record/305796
ER  -