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000306227 041__ $$aEnglish
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000306227 1001_ $$00000-0003-0423-7825$$aKotzur, Rebecca$$b0
000306227 245__ $$aNK-92 cells retain vitality and functionality when grown in standard cell culture conditions.
000306227 260__ $$aSan Francisco, California, US$$bPLOS$$c2022
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000306227 520__ $$aNK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most 'NK-like' cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies.
000306227 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000306227 650_7 $$2NLM Chemicals$$aCulture Media
000306227 650_2 $$2MeSH$$aCell Culture Techniques
000306227 650_2 $$2MeSH$$aCulture Media: metabolism
000306227 650_2 $$2MeSH$$aCytotoxicity, Immunologic
000306227 650_2 $$2MeSH$$aHumans
000306227 650_2 $$2MeSH$$aImmunotherapy
000306227 650_2 $$2MeSH$$aKiller Cells, Natural: metabolism
000306227 650_2 $$2MeSH$$aNeoplasms: metabolism
000306227 650_2 $$2MeSH$$aNeoplasms: therapy
000306227 7001_ $$aDuev-Cohen, Alexandra$$b1
000306227 7001_ $$aKol, Inbal$$b2
000306227 7001_ $$aReches, Adi$$b3
000306227 7001_ $$aMandelboim, Ofer$$b4
000306227 7001_ $$aStein, Natan$$b5
000306227 773__ $$0PERI:(DE-600)2267670-3$$a10.1371/journal.pone.0264897$$gVol. 17, no. 3, p. e0264897 -$$n3$$pe0264897$$tPLOS ONE$$v17$$x1932-6203$$y2022
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