TY  - JOUR
AU  - Kotzur, Rebecca
AU  - Duev-Cohen, Alexandra
AU  - Kol, Inbal
AU  - Reches, Adi
AU  - Mandelboim, Ofer
AU  - Stein, Natan
TI  - NK-92 cells retain vitality and functionality when grown in standard cell culture conditions.
JO  - PLOS ONE
VL  - 17
IS  - 3
SN  - 1932-6203
CY  - San Francisco, California, US
PB  - PLOS
M1  - DKFZ-2025-02456
SP  - e0264897
PY  - 2022
N1  - #DKFZ-MOST-Ca186#
AB  - NK-92 cells are an off-the-shelf, cell-based immunotherapy currently in clinical trials for a variety of cancer types. As the most 'NK-like' cell line available, it is also an important research tool. To date, NK-92 cells have been cultivated in a costly and time-consumingly prepared specialized medium, complicating research with these cells. Here we show that NK-92 cells grow in the comparatively user-friendly RPMI medium supplemented with IL-2. We demonstrate that their metabolic activity and replication rates are even improved in RPMI. Furthermore, they can be grown in cell culture dishes and do not need to be expanded in ventilated flasks. We show that in RPMI the cells retain functional characteristics relating to receptor expression, IFN-γ secretion, and killing. Our findings will enable more researchers to work with and manipulate this cell line, hopefully leading to further discoveries and improved therapies.
KW  - Cell Culture Techniques
KW  - Culture Media: metabolism
KW  - Cytotoxicity, Immunologic
KW  - Humans
KW  - Immunotherapy
KW  - Killer Cells, Natural: metabolism
KW  - Neoplasms: metabolism
KW  - Neoplasms: therapy
KW  - Culture Media (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:35294457
C2  - pmc:PMC8926178
DO  - DOI:10.1371/journal.pone.0264897
UR  - https://inrepo02.dkfz.de/record/306227
ER  -