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@ARTICLE{FalickMichaeli:306269,
author = {T. Falick Michaeli and O. Sabag and B. Azria and R. Fok and
N. Abudi and R. Abramovitch and J. Monin and Y. Gielchinsky
and H. Cedar and Y. Bergman},
title = {{H}epatocyte regeneration is driven by embryo-like {DNA}
methylation reprogramming.},
journal = {Proceedings of the National Academy of Sciences of the
United States of America},
volume = {121},
number = {16},
issn = {0027-8424},
address = {Washington, DC},
publisher = {National Acad. of Sciences},
reportid = {DKFZ-2025-02495},
pages = {e2314885121},
year = {2024},
note = {#DKFZ-MOST-Ca200#},
abstract = {As a result of partial hepatectomy, the remaining liver
tissue undergoes a process of renewed proliferation that
leads to rapid regeneration of the liver. By following the
early stages of this process, we observed dramatic
programmed changes in the DNA methylation profile,
characterized by both de novo and demethylation events, with
a subsequent return to the original adult pattern as the
liver matures. Strikingly, these transient alterations
partially mimic the DNA methylation state of embryonic
hepatoblasts (E16.5), indicating that hepatocytes actually
undergo epigenetic dedifferentiation. Furthermore,
Tet2/Tet3-deletion experiments demonstrated that these
changes in methylation are necessary for carrying out basic
embryonic functions, such as proliferation, a key step in
liver regeneration. This implies that unlike tissue-specific
regulatory regions that remain demethylated in the adult,
early embryonic genes are programmed to first undergo
demethylation, followed by remethylation as development
proceeds. The identification of this built-in system may
open targeting opportunities for regenerative medicine.},
keywords = {DNA Methylation / Embryo, Mammalian: metabolism /
Hepatocytes / DNA methylation (Other) / dedifferentiation
(Other) / partial hepatectomy (Other)},
ddc = {500},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:38588413},
pmc = {pmc:PMC11032470},
doi = {10.1073/pnas.2314885121},
url = {https://inrepo02.dkfz.de/record/306269},
}