000306280 001__ 306280
000306280 005__ 20251119103400.0
000306280 0247_ $$2doi$$a10.1038/s41467-025-60430-z
000306280 0247_ $$2pmid$$apmid:40461574
000306280 0247_ $$2pmc$$apmc:PMC12134209
000306280 037__ $$aDKFZ-2025-02506
000306280 041__ $$aEnglish
000306280 082__ $$a500
000306280 1001_ $$00000-0002-1514-6366$$aArfè, S.$$b0
000306280 245__ $$aH3.3 deposition counteracts the replication-dependent enrichment of H3.1 at chromocenters in embryonic stem cells.
000306280 260__ $$a[London]$$bSpringer Nature$$c2025
000306280 3367_ $$2DRIVER$$aarticle
000306280 3367_ $$2DataCite$$aOutput Types/Journal article
000306280 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1763544781_2172554
000306280 3367_ $$2BibTeX$$aARTICLE
000306280 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000306280 3367_ $$00$$2EndNote$$aJournal Article
000306280 500__ $$a#DKFZ-MOST-Ca215#
000306280 520__ $$aChromocenters in mouse cells are membrane-less nuclear compartments representing typical heterochromatin stably maintained during cell cycle. We explore how histone H3 variants, replicative H3.1/2 or replacement H3.3, mark these domains during the cell cycle in mouse embryonic stem cells, neuronal precursor cells as well as immortalized 3T3 cells. We find a strong and distinct H3.1 enrichment at chromocenters, with variation in mouse embryonic stem cells. Mechanistically, this H3.1 selective enrichment depends on the DNA Synthesis Coupled deposition pathway operating in S phase challenged when we target H3.3 deposition through the DNA Synthesis Independent deposition pathway mediated by HIRA. Altering the H3.1/H3.3 dynamics at chromocenters in mouse embryonic stem cells affects nuclear morphology and cell division. Here, we reveal opposing mechanisms for H3.1 and H3.3 deposition with different enforcement according to cell cycle and potency which determine their ratio at chromocenters and are critical for genome stability and cell survival.
000306280 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000306280 650_7 $$2NLM Chemicals$$aHistones
000306280 650_7 $$2NLM Chemicals$$aHeterochromatin
000306280 650_2 $$2MeSH$$aAnimals
000306280 650_2 $$2MeSH$$aHistones: metabolism
000306280 650_2 $$2MeSH$$aHistones: genetics
000306280 650_2 $$2MeSH$$aMice
000306280 650_2 $$2MeSH$$aHeterochromatin: metabolism
000306280 650_2 $$2MeSH$$aMouse Embryonic Stem Cells: metabolism
000306280 650_2 $$2MeSH$$aMouse Embryonic Stem Cells: cytology
000306280 650_2 $$2MeSH$$aDNA Replication
000306280 650_2 $$2MeSH$$aCell Cycle
000306280 650_2 $$2MeSH$$aEmbryonic Stem Cells: metabolism
000306280 650_2 $$2MeSH$$aCell Nucleus: metabolism
000306280 650_2 $$2MeSH$$a3T3 Cells
000306280 7001_ $$00000-0002-5923-5678$$aKaragyozova, T.$$b1
000306280 7001_ $$aForest, A.$$b2
000306280 7001_ $$00009-0001-8831-5175$$aBingham, D.$$b3
000306280 7001_ $$aHmidan, H.$$b4
000306280 7001_ $$00000-0003-3998-9330$$aMazaud, D.$$b5
000306280 7001_ $$00000-0002-3999-0879$$aGarnier, M.$$b6
000306280 7001_ $$00000-0001-8650-4995$$aLe Baccon, P.$$b7
000306280 7001_ $$00000-0003-4777-986X$$aMeshorer, E.$$b8
000306280 7001_ $$00000-0001-6557-7204$$aQuivy, J-P$$b9
000306280 7001_ $$00000-0001-5570-0723$$aAlmouzni, G.$$b10
000306280 773__ $$0PERI:(DE-600)2553671-0$$a10.1038/s41467-025-60430-z$$gVol. 16, no. 1, p. 5138$$n1$$p5138$$tNature Communications$$v16$$x2041-1723$$y2025
000306280 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNAT COMMUN : 2022$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-01-30T07:48:07Z
000306280 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-01-30T07:48:07Z
000306280 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Peer review$$d2024-01-30T07:48:07Z
000306280 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ$$d2024-01-30T07:48:07Z
000306280 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)9915$$2StatID$$aIF >= 15$$bNAT COMMUN : 2022$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2025-01-02
000306280 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2025-01-02
000306280 980__ $$ajournal
000306280 980__ $$aI:(DE-He78)W500-20160331
000306280 980__ $$aI:(DE-He78)W510-20160331
000306280 9801_ $$aEXTERN4COORD