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@ARTICLE{Erez:306282,
      author       = {N. Erez and N. Furth and V. Fedyuk and J. Wadden and R.
                      Aittaleb and T. Adam and K. Schwark and M. Niculcea and M.
                      Miclea and R. Mody and A. Franson and H. A. Parmar and M.
                      Ibrahim and B. Lau and A. Eze and N. Nourmohammadi and I.
                      Fried and J. Nazarian and G. Ron and S. Venneti and C.
                      Koschmann and E. Shema},
      title        = {{S}ingle-molecule systems for the detection and monitoring
                      of plasma-circulating nucleosomes and oncoproteins in
                      diffuse midline glioma.},
      journal      = {Cell reports / Medicine},
      volume       = {6},
      number       = {1},
      issn         = {2666-3791},
      address      = {Cambridge, MA},
      publisher    = {Cell Press},
      reportid     = {DKFZ-2025-02508},
      pages        = {101918},
      year         = {2025},
      note         = {#DKFZ-MOST-Ca219#},
      abstract     = {The analysis of cell-free tumor DNA (ctDNA) and proteins in
                      the blood of patients with cancer potentiates a new
                      generation of non-invasive diagnostic approaches. However,
                      confident detection of tumor-originating markers is
                      challenging, especially in the context of brain tumors,
                      where these analytes in plasma are extremely scarce. Here,
                      we apply a sensitive single-molecule technology to profile
                      multiple histone modifications on individual nucleosomes
                      from the plasma of patients with diffuse midline glioma
                      (DMG). The system reveals epigenetic patterns unique to DMG,
                      significantly differentiating this group of patients from
                      healthy subjects or individuals diagnosed with other cancer
                      types. We further develop a method to directly quantify the
                      tumor-originating oncoproteins, lysine 27 to methionine
                      substitution in histone H3 (H3-K27M) and mutant p53, from <1
                      mL of plasma, allowing for the accurate molecular
                      classification of patients with DMG. We show that our
                      strategy correlates with MRI and droplet-digital PCR (ddPCR)
                      measurements of ctDNA, highlighting the clinical potential
                      of single-molecule-based, multi-parametric assays for DMG
                      diagnosis and treatment monitoring.},
      keywords     = {Humans / Nucleosomes: metabolism / Glioma: blood / Glioma:
                      genetics / Glioma: diagnosis / Glioma: pathology / Histones:
                      genetics / Histones: blood / Histones: metabolism / Brain
                      Neoplasms: blood / Brain Neoplasms: genetics / Brain
                      Neoplasms: diagnosis / Biomarkers, Tumor: blood /
                      Biomarkers, Tumor: genetics / Male / Female / Circulating
                      Tumor DNA: blood / Tumor Suppressor Protein p53: blood /
                      Tumor Suppressor Protein p53: genetics / Single Molecule
                      Imaging: methods / Adult / Middle Aged / DIPG (Other) /
                      H3-K27M (Other) / TP53 (Other) / liquid-biopsy (Other) /
                      oncohistones (Other) / single-molecule (Other) / Nucleosomes
                      (NLM Chemicals) / Histones (NLM Chemicals) / Biomarkers,
                      Tumor (NLM Chemicals) / Circulating Tumor DNA (NLM
                      Chemicals) / Tumor Suppressor Protein p53 (NLM Chemicals)},
      ddc          = {610},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39809263},
      pmc          = {pmc:PMC11866549},
      doi          = {10.1016/j.xcrm.2024.101918},
      url          = {https://inrepo02.dkfz.de/record/306282},
}