000306284 001__ 306284
000306284 005__ 20251119104132.0
000306284 0247_ $$2doi$$a10.3390/ijms26072988
000306284 0247_ $$2pmid$$apmid:40243610
000306284 0247_ $$2pmc$$apmc:PMC11988885
000306284 0247_ $$2ISSN$$a1422-0067
000306284 0247_ $$2ISSN$$a1661-6596
000306284 037__ $$aDKFZ-2025-02510
000306284 041__ $$aEnglish
000306284 082__ $$a540
000306284 1001_ $$aYassin, Abed Al-Kader$$b0
000306284 245__ $$aEnhancing the Efficacy of CAR-T Cell Production Using BX795 and Rosuvastatin in a Serum-Free Medium.
000306284 260__ $$aBasel$$bMolecular Diversity Preservation International$$c2025
000306284 3367_ $$2DRIVER$$aarticle
000306284 3367_ $$2DataCite$$aOutput Types/Journal article
000306284 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1763545263_2172555
000306284 3367_ $$2BibTeX$$aARTICLE
000306284 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000306284 3367_ $$00$$2EndNote$$aJournal Article
000306284 500__ $$a#DKFZ-MOST-Ca222#
000306284 520__ $$aChimeric Antigen Receptor T-cell (CAR-T) therapy has emerged as a transformative approach for cancer treatment, demonstrating remarkable success in patients with relapsed and refractory hematological malignancies. However, challenges persist in optimizing CAR-T cell production and improving therapeutic outcomes. One of the major hurdles is the efficiency of retroviral or lentiviral transduction during CAR-T cell manufacturing. Additionally, the heterogeneity of T-cell populations isolated from patients can impact CAR-T cell effectiveness and persistence in vivo. This article explores a novel strategy to address these challenges by focusing on serum-free medium and additive optimization. We propose a unique approach that incorporates the culturing of T cells in Nutri-T medium, along with 24 h of exposure to combined low concentrations of BX795 and rosuvastatin, to enhance the transduction efficacy and functionality of CAR-T cells. The results presented here provide promising insights into the potential of this strategy to produce more effective CAR-T cells for immunotherapy, ultimately advancing the field and benefiting cancer patients worldwide.
000306284 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo02.dkfz.de
000306284 650_7 $$2Other$$aBX795
000306284 650_7 $$2Other$$aCAR-T
000306284 650_7 $$2Other$$aNutri-T
000306284 650_7 $$2Other$$aPBMCs
000306284 650_7 $$2Other$$alentivirus
000306284 650_7 $$2Other$$arosuvastatin
000306284 650_7 $$083MVU38M7Q$$2NLM Chemicals$$aRosuvastatin Calcium
000306284 650_7 $$2NLM Chemicals$$aReceptors, Chimeric Antigen
000306284 650_7 $$2NLM Chemicals$$aCulture Media, Serum-Free
000306284 650_2 $$2MeSH$$aHumans
000306284 650_2 $$2MeSH$$aRosuvastatin Calcium: pharmacology
000306284 650_2 $$2MeSH$$aReceptors, Chimeric Antigen: metabolism
000306284 650_2 $$2MeSH$$aReceptors, Chimeric Antigen: immunology
000306284 650_2 $$2MeSH$$aReceptors, Chimeric Antigen: genetics
000306284 650_2 $$2MeSH$$aImmunotherapy, Adoptive: methods
000306284 650_2 $$2MeSH$$aT-Lymphocytes: drug effects
000306284 650_2 $$2MeSH$$aT-Lymphocytes: immunology
000306284 650_2 $$2MeSH$$aT-Lymphocytes: metabolism
000306284 650_2 $$2MeSH$$aCulture Media, Serum-Free: pharmacology
000306284 7001_ $$aBanerji, Rajashri$$b1
000306284 7001_ $$00000-0003-3399-7399$$aBhattacharya, Baisali$$b2
000306284 7001_ $$00009-0001-7282-0257$$aRadinsky, Olga$$b3
000306284 7001_ $$00000-0002-7643-1275$$aHadad, Uzi$$b4
000306284 7001_ $$00000-0001-7740-0473$$aKaufman, Bar$$b5
000306284 7001_ $$aPorgador, Angel$$b6
000306284 773__ $$0PERI:(DE-600)2019364-6$$a10.3390/ijms26072988$$gVol. 26, no. 7, p. 2988 -$$n7$$p2988$$tInternational journal of molecular sciences$$v26$$x1422-0067$$y2025
000306284 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-21
000306284 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-21
000306284 980__ $$ajournal
000306284 980__ $$aI:(DE-He78)W500-20160331
000306284 980__ $$aI:(DE-He78)W510-20160331
000306284 9801_ $$aEXTERN4COORD