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@ARTICLE{Volckmar:119224,
author = {A.-L. Volckmar and J. Leichsenring and C. Flechtenmacher
and N. Pfarr and U. Siebolts and M. Kirchner and J. Budczies
and M. Bockmayr and K. Ridinger and K. Lorenz and E.
Herpel$^*$ and A. Noske and W. Weichert$^*$ and F. Klauschen
and P. Schirmacher$^*$ and R. Penzel and V. Endris and A.
Stenzinger$^*$},
title = {{T}ubular, lactating, and ductal adenomas are devoid of
{MED}12 {E}xon2 mutations, and ductal adenomas show
recurrent mutations in {GNAS} and the {PI}3{K}-{AKT}
pathway},
journal = {Genes, chromosomes $\&$ cancer},
volume = {56},
number = {1},
issn = {1045-2257},
address = {New York, NY},
publisher = {Wiley-Liss},
reportid = {DKFZ-2017-00014},
pages = {11 - 17},
year = {2017},
abstract = {Adenomas of the breast are rare benign tumors although
single cases with malignant behavior have been reported.
However, the genetic basis of these tumors is unknown.
Employing targeted next generation sequencing of 50
cancer-related genes as well as Sanger sequencing, we
profiled a cohort of 18 mammary adenomas comprising 9
ductal, 6 tubular, and 3 lactating adenoma. Missense
mutations were detected in 8 of the 18 cases $(44\%).$
Specifically, five $(56\%)$ ductal adenomas and three
$(50\%)$ tubular adenomas harbored mutated genes. No
mutations were detected in lactating adenomas. Three of the
nine ductal adenomas showed mutant AKT1 (p.E17K) with two of
them harboring an additional GNAS mutation (p.R201C). One
case had mutant PIK3CA (p.H1047R) and another case a
mutation in GNAS (p.R201C). The three cases of mutated
tubular adenomas showed mutations in either MET or FGFR3. Of
note, we did not detect copy number changes and none of the
cases including tubular adenomas had mutations in exon 2 of
MED12. Our results suggest that ductal adenomas are related
to papillomas of the breast and screening for mutations in
exon 2 of MED12 might help to facilitate differential
diagnosis between tubular adenoma and fibroadenoma in
difficult cases. Lastly, our data exemplarily demonstrate
that mutations in cancer-related genes per se do not
indicate malignancy but occur in benign tumors. © 2016
Wiley Periodicals, Inc.},
cin = {G010 / L101 / L701},
ddc = {570},
cid = {I:(DE-He78)G010-20160331 / I:(DE-He78)L101-20160331 /
I:(DE-He78)L701-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:27438523},
doi = {10.1002/gcc.22396},
url = {https://inrepo02.dkfz.de/record/119224},
}