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@ARTICLE{Unsay:119256,
author = {J. D. Unsay$^*$ and K. Cosentino and K. Sporbeck and A. J.
García-Sáez},
title = {{P}ro-apoptotic c{B}id and {B}ax exhibit distinct membrane
remodeling activities: {A}n {AFM} study.},
journal = {Biochimica et biophysica acta / Biomembranes},
volume = {1859},
number = {1},
issn = {0005-2736},
address = {Amsterdam},
publisher = {Elsevier},
reportid = {DKFZ-2017-00042},
pages = {17 - 27},
year = {2017},
abstract = {Bcl-2 proteins are key regulators of the mitochondrial
outer membrane (MOM) permeabilization that mediates
apoptosis. During apoptosis, Bid is cleaved (cBid) and
translocates to the MOM, where it activates Bax. Bax then
oligomerizes and induces MOM permeabilization. However,
little is known about how these proteins affect membrane
organization aside from pore formation. In previous studies,
we have shown that both cBid and Bax are able to remodel
membranes and stabilize curvature. Here, we dissected the
independent effects of Bax and cBid on supported lipid
structures mimicking the mitochondrial composition by means
of atomic force spectroscopy. We show that cBid did not
permeabilize the membrane but lowered the membrane
breakthrough force. On the other hand, Bax effects were
dependent on its oligomeric state. Monomeric Bax did not
affect the membrane properties. In contrast, oligomeric Bax
lowered the breakthrough force of the membrane, which in the
context of pore formation, implies a lowering of the line
tension at the edge of the pore.},
cin = {B160},
ddc = {570},
cid = {I:(DE-He78)B160-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:27755971},
doi = {10.1016/j.bbamem.2016.10.007},
url = {https://inrepo02.dkfz.de/record/119256},
}