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@ARTICLE{Dieter:119323,
author = {S. Dieter$^*$ and K. Giessler$^*$ and M. Kriegsmann and T.
D. Dubash$^*$ and L. Möhrmann$^*$ and E. Schulz$^*$ and C.
Siegl$^*$ and S. Weber$^*$ and H. Strakerjahn$^*$ and A.
Oberlack$^*$ and U. Heger and J. Gao$^*$ and E.-M.
Hartinger$^*$ and F. Oppel$^*$ and C. M. Hoffmann and N.
Ha$^*$ and B. Brors$^*$ and F. Lasitschka and A. Ulrich and
O. Strobel and M. Schmidt$^*$ and C. von Kalle$^*$ and M.
Schneider and W. Weichert$^*$ and K. R. Ehrenberg$^*$ and H.
Glimm$^*$ and C. Ball$^*$},
title = {{P}atient-derived xenografts of gastrointestinal cancers
are susceptible to rapid and delayed
{B}-lymphoproliferation.},
journal = {International journal of cancer},
volume = {140},
number = {6},
issn = {0020-7136},
address = {Bognor Regis},
publisher = {Wiley-Liss},
reportid = {DKFZ-2017-00078},
pages = {1356 - 1363},
year = {2017},
abstract = {Patient-derived cancer xenografts (PDX) are widely used to
identify and evaluate novel therapeutic targets, and to test
therapeutic approaches in preclinical mouse avatar trials.
Despite their widespread use, potential caveats of PDX
models remain considerably underappreciated. Here, we
demonstrate that EBV-associated B-lymphoproliferations
frequently develop following xenotransplantation of human
colorectal and pancreatic carcinomas in highly
immunodeficient NOD.Cg-Prkdc(scid) Il2rg(tm1Wjl) /SzJ (NSG)
mice (18/47 and 4/37 mice, respectively), and in derived
cell cultures in vitro. Strikingly, even PDX with carcinoma
histology can host scarce EBV-infected B-lymphocytes that
can fully overgrow carcinoma cells during serial passaging
in vitro and in vivo. As serial xenografting is crucial to
expand primary tumor tissue for biobanks and cohorts for
preclinical mouse avatar trials, the emerging dominance of
B-lymphoproliferations in serial PDX represents a serious
confounding factor in these models. Consequently, repeated
phenotypic assessments of serial PDX are mandatory at each
expansion step to verify 'bona fide' carcinoma xenografts.},
cin = {G010 / G100 / G200 / M110 / L101},
ddc = {610},
cid = {I:(DE-He78)G010-20160331 / I:(DE-He78)G100-20160331 /
I:(DE-He78)G200-20160331 / I:(DE-He78)M110-20160331 /
I:(DE-He78)L101-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:27935045},
doi = {10.1002/ijc.30561},
url = {https://inrepo02.dkfz.de/record/119323},
}