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@ARTICLE{Herzig:119335,
author = {Y. Herzig and S. Nevo and C. Bornstein and M. R. Brezis and
S. Ben-Hur and A. Shkedy and M. Eisenberg-Bord and B. Levi
and M. Delacher$^*$ and Y. Goldfarb and E. David and L.
Weinberger and S. Viukov and S. Ben-Dor and M. Giraud and J.
H. Hanna and A. Breiling$^*$ and F. Lyko$^*$ and I. Amit and
M. Feuerer$^*$ and J. Abramson},
title = {{T}ranscriptional programs that control expression of the
autoimmune regulator gene {A}ire.100},
journal = {Nature immunology},
volume = {18},
number = {2},
issn = {1529-2916},
address = {New York, NY},
publisher = {Nature America Inc.},
reportid = {DKFZ-2017-00090},
pages = {161 - 172},
year = {2017},
abstract = {Aire is a transcriptional regulator that induces
promiscuous expression of thousands of genes encoding
tissue-restricted antigens (TRAs) in medullary thymic
epithelial cells (mTECs). While the target genes of Aire are
well characterized, the transcriptional programs that
regulate its own expression have remained elusive. Here we
comprehensively analyzed both cis-acting and trans-acting
regulatory mechanisms and found that the Aire locus was
insulated by the global chromatin organizer CTCF and was
hypermethylated in cells and tissues that did not express
Aire. In mTECs, however, Aire expression was facilitated by
concurrent eviction of CTCF, specific demethylation of exon
2 and the proximal promoter, and the coordinated action of
several transcription activators, including Irf4, Irf8,
Tbx21, Tcf7 and Ctcfl, which acted on mTEC-specific
accessible regions in the Aire locus.},
cin = {A130 / D100},
ddc = {610},
cid = {I:(DE-He78)A130-20160331 / I:(DE-He78)D100-20160331},
pnm = {314 - Tumor immunology (POF3-314)},
pid = {G:(DE-HGF)POF3-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:27941786},
doi = {10.1038/ni.3638},
url = {https://inrepo02.dkfz.de/record/119335},
}