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001     119665
005     20240228134948.0
024 7 _ |a 10.1038/srep07531
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037 _ _ |a DKFZ-2017-00296
041 _ _ |a eng
082 _ _ |a 000
100 1 _ |a Blasche, Sonja
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|e First author
245 _ _ |a The EHEC-host interactome reveals novel targets for the translocated intimin receptor.
260 _ _ |a London
|c 2014
|b Nature Publishing Group
336 7 _ |a article
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336 7 _ |a ARTICLE
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520 _ _ |a Enterohemorrhagic E. coli (EHEC) manipulate their human host through at least 39 effector proteins which hijack host processes through direct protein-protein interactions (PPIs). To identify their protein targets in the host cells, we performed yeast two-hybrid screens, allowing us to find 48 high-confidence protein-protein interactions between 15 EHEC effectors and 47 human host proteins. In comparison to other bacteria and viruses we found that EHEC effectors bind more frequently to hub proteins as well as to proteins that participate in a higher number of protein complexes. The data set includes six new interactions that involve the translocated intimin receptor (TIR), namely HPCAL1, HPCAL4, NCALD, ARRB1, PDE6D, and STK16. We compared these TIR interactions in EHEC and enteropathogenic E. coli (EPEC) and found that five interactions were conserved. Notably, the conserved interactions included those of serine/threonine kinase 16 (STK16), hippocalcin-like 1 (HPCAL1) as well as neurocalcin-delta (NCALD). These proteins co-localize with the infection sites of EPEC. Furthermore, our results suggest putative functions of poorly characterized effectors (EspJ, EspY1). In particular, we observed that EspJ is connected to the microtubule system while EspY1 appears to be involved in apoptosis/cell cycle regulation.
536 _ _ |a 312 - Functional and structural genomics (POF3-312)
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588 _ _ |a Dataset connected to CrossRef, PubMed,
650 _ 7 |a Adhesins, Bacterial
|2 NLM Chemicals
650 _ 7 |a Escherichia coli Proteins
|2 NLM Chemicals
650 _ 7 |a EspJ protein, E coli
|2 NLM Chemicals
650 _ 7 |a HPCAL1 protein, human
|2 NLM Chemicals
650 _ 7 |a NCALD protein, human
|2 NLM Chemicals
650 _ 7 |a Neurocalcin
|2 NLM Chemicals
650 _ 7 |a Receptors, Cell Surface
|2 NLM Chemicals
650 _ 7 |a Tir protein, E coli
|2 NLM Chemicals
650 _ 7 |a Transcription Factors
|2 NLM Chemicals
650 _ 7 |a eaeA protein, E coli
|0 147094-99-3
|2 NLM Chemicals
650 _ 7 |a Protein-Serine-Threonine Kinases
|0 EC 2.7.11.1
|2 NLM Chemicals
650 _ 7 |a STK16 protein, human
|0 EC 2.7.11.1
|2 NLM Chemicals
700 1 _ |a Arens, Stefan
|b 1
700 1 _ |a Ceol, Arnaud
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700 1 _ |a Siszler, Gabriella
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700 1 _ |a Schmidt, M Alexander
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700 1 _ |a Häuser, Roman
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700 1 _ |a Schwarz, Frank
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700 1 _ |a Wuchty, Stefan
|b 7
700 1 _ |a Aloy, Patrick
|b 8
700 1 _ |a Uetz, Peter
|b 9
700 1 _ |a Stradal, Theresia
|b 10
700 1 _ |a Koegl, Manfred
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|e Last author
773 _ _ |a 10.1038/srep07531
|g Vol. 4, p. 7531 -
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|y 2014
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