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@ARTICLE{Balzarotti:119827,
author = {F. Balzarotti and Y. Eilers and K. C. Gwosch and A. H.
Gynnå and V. Westphal and F. D. Stefani and J. Elf and S.
Hell$^*$},
title = {{N}anometer resolution imaging and tracking of fluorescent
molecules with minimal photon fluxes.},
journal = {Science},
volume = {355},
number = {6325},
issn = {1095-9203},
address = {Washington, DC [u.a.]},
publisher = {American Association for the Advancement of Science38566},
reportid = {DKFZ-2017-00422},
pages = {606 - 612},
year = {2017},
abstract = {We introduce MINFLUX, a concept for localizing photon
emitters in space. By probing the emitter with a local
intensity minimum of excitation light, MINFLUX minimizes the
fluorescence photons needed for high localization precision.
In our experiments, 22 times fewer fluorescence photons are
required as compared to popular centroid localization. In
superresolution microscopy, MINFLUX attained ~1-nm
precision, resolving molecules only 6 nanometers apart.
MINFLUX tracking of single fluorescent proteins increased
the temporal resolution and the number of localizations per
trace by a factor of 100, as demonstrated with diffusing 30S
ribosomal subunits in living Escherichia coli As conceptual
limits have not been reached, we expect this localization
modality to break new ground for observing the dynamics,
distribution, and structure of macromolecules in living
cells and beyond.},
cin = {E190},
ddc = {500},
cid = {I:(DE-He78)E190-20160331},
pnm = {315 - Imaging and radiooncology (POF3-315)},
pid = {G:(DE-HGF)POF3-315},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28008086},
doi = {10.1126/science.aak9913},
url = {https://inrepo02.dkfz.de/record/119827},
}