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000119865 1001_ $$0P:(DE-He78)d6da8152e37bbf1d9843a7a213d99a30$$aDepner, Sofia$$b0$$eFirst author$$udkfz
000119865 245__ $$aCell type specific interleukin-6 induced responses in tumor keratinocytes and stromal fibroblasts are essential for invasive growth.
000119865 260__ $$aBognor Regis$$bWiley-Liss$$c2014
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000119865 520__ $$aInterleukin-6 (IL-6) is one of the major inflammatory interleukins that has been linked to cancer progression. In our model for human skin squamous cell carcinoma (SCC), IL-6 expression is strongly upregulated upon progression from benign tumors to highly malignant, metastasizing SCCs. We now demonstrate that IL-6 promotes malignant and invasive tumor growth in human skin SCCs by inducing cell type specific cytokine profiles in tumor keratinocytes and stromal fibroblasts, activating the latter towards a tumor associated fibroblast (TAF) phenotype. In three-dimensional organotypic cocultures in vitro invasive growth of IL-6 overexpressing tumor keratinocytes, is associated with increased expression of matrix metalloproteinase-2 (MMP-2), MMP-14 and tissue inhibitor of metalloproteinases-2, and clearly depends on IL-6 activated fibroblasts. IL-6-induced secretion of monocyte chemotactic protein-1 (MCP-1) in tumor keratinocytes and of hepatocyte growth factor in fibroblasts is crucial for regulating expression and activation of MMP-2. This functional role of IL-6 is confirmed in vivo. Here MMP-14 and MMP-2 expression occur exclusively in surface transplants of IL-6 overexpressing keratinocytes and fibroblasts are identified as important source of MMP-2. Our data indicate that tumor keratinocytes derived IL-6 activates stromal fibroblasts towards a TAF phenotype, promoting tumor invasion via enhanced expression and activation of MMP-2.
000119865 536__ $$0G:(DE-HGF)POF3-311$$a311 - Signalling pathways, cell and tumor biology (POF3-311)$$cPOF3-311$$fPOF III$$x0
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000119865 650_7 $$2NLM Chemicals$$aCulture Media, Conditioned
000119865 650_7 $$2NLM Chemicals$$aInterleukin-6
000119865 650_7 $$0EC 3.4.24.24$$2NLM Chemicals$$aMMP2 protein, human
000119865 650_7 $$0EC 3.4.24.24$$2NLM Chemicals$$aMatrix Metalloproteinase 2
000119865 7001_ $$aLederle, Wiltrud$$b1
000119865 7001_ $$0P:(DE-HGF)0$$aGutschalk, Claudia$$b2
000119865 7001_ $$0P:(DE-HGF)0$$aLinde, Nina$$b3
000119865 7001_ $$0P:(DE-HGF)0$$aZajonz, Alexandra$$b4
000119865 7001_ $$0P:(DE-HGF)0$$aMueller, Margareta M$$b5$$eLast author
000119865 773__ $$0PERI:(DE-600)1474822-8$$gVol. 135, no. 3$$n3$$p551-562$$tInternational journal of cancer$$v135$$x0020-7136$$y2014
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000119865 9141_ $$y2014
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