Home > Publications database > Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins. > print

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024 7 _ |a 10.1128/MCB.00766-14
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024 7 _ |a 0270-7306
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024 7 _ |a 1098-5549
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037 _ _ |a DKFZ-2017-00600
041 _ _ |a eng
082 _ _ |a 570
100 1 _ |a Fischer-Kešo, Regina
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|e First author
245 _ _ |a Plakophilins 1 and 3 bind to FXR1 and thereby influence the mRNA stability of desmosomal proteins.
260 _ _ |a Washington, DC
|c 2014
|b Soc.
336 7 _ |a article
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336 7 _ |a Journal Article
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520 _ _ |a Plakophilins 1 and 3 (PKP1/3) are members of the arm repeat family of catenin proteins and serve as structural components of desmosomes, which are important for cell-cell-adhesion. In addition, PKP1/3 occur as soluble proteins outside desmosomes, yet their role in the cytoplasm is not known. We found that cytoplasmic PKP1/3 coprecipitated with the RNA-binding proteins FXR1, G3BP, PABPC1, and UPF1, and these PKP1/3 complexes also comprised desmoplakin and PKP2 mRNAs. Moreover, we showed that the interaction of PKP1/3 with G3BP, PABPC1, and UPF1 but not with FXR1 was RNase sensitive. To address the cytoplasmic function of PKP1/3, we performed gain-and-loss-of-function studies. Both PKP1 and PKP3 knockdown cell lines showed reduced protein and mRNA levels for desmoplakin and PKP2. Whereas global rates of translation were unaffected, desmoplakin and PKP2 mRNA were destabilized. Furthermore, binding of PKP1/3 to FXR1 was RNA independent, and both PKP3 and FXR1 stabilized PKP2 mRNA. Our results demonstrate that cytoplasmic PKP1/3 are components of mRNA ribonucleoprotein particles and act as posttranscriptional regulators of gene expression.
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650 _ 7 |a Carrier Proteins
|2 NLM Chemicals
650 _ 7 |a Desmoplakins
|2 NLM Chemicals
650 _ 7 |a FXR1 protein, human
|2 NLM Chemicals
650 _ 7 |a G3BP protein, human
|2 NLM Chemicals
650 _ 7 |a PABPC1L protein, human
|2 NLM Chemicals
650 _ 7 |a PKP1 protein, human
|2 NLM Chemicals
650 _ 7 |a PKP3 protein, human
|2 NLM Chemicals
650 _ 7 |a Plakophilins
|2 NLM Chemicals
650 _ 7 |a Poly(A)-Binding Proteins
|2 NLM Chemicals
650 _ 7 |a RNA, Messenger
|2 NLM Chemicals
650 _ 7 |a RNA-Binding Proteins
|2 NLM Chemicals
650 _ 7 |a Trans-Activators
|2 NLM Chemicals
650 _ 7 |a UPF1 protein, human
|2 NLM Chemicals
700 1 _ |a Breuninger, Sonja
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Hofmann, Sarah
|0 P:(DE-HGF)0
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700 1 _ |a Henn, Manuela
|0 P:(DE-HGF)0
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700 1 _ |a Röhrig, Theresa
|b 4
700 1 _ |a Ströbel, Philipp
|b 5
700 1 _ |a Stoecklin, Georg
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700 1 _ |a Hofmann, Ilse
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773 _ _ |a 10.1128/MCB.00766-14
|g Vol. 34, no. 23, p. 4244 - 4256
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|t Molecular and cellular biology
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