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@ARTICLE{Huser:120175,
author = {R. Häuser$^*$ and A. Ceol and S. V. Rajagopala and R.
Mosca and G. Siszler and N. Wermke$^*$ and P. Sikorski and
F. Schwarz$^*$ and M. Schick$^*$ and S. Wuchty and P. Aloy
and P. Uetz},
title = {{A} second-generation protein-protein interaction network
of {H}elicobacter pylori.},
journal = {Molecular $\&$ cellular proteomics},
volume = {13},
number = {5},
issn = {1535-9484},
address = {Bethesda, Md.},
publisher = {The American Society for Biochemistry and Molecular
Biology},
reportid = {DKFZ-2017-00757},
pages = {1318 - 1329},
year = {2014},
abstract = {Helicobacter pylori infections cause gastric ulcers and
play a major role in the development of gastric cancer. In
2001, the first protein interactome was published for this
species, revealing over 1500 binary protein interactions
resulting from 261 yeast two-hybrid screens. Here we roughly
double the number of previously published interactions using
an ORFeome-based, proteome-wide yeast two-hybrid screening
strategy. We identified a total of 1515 protein-protein
interactions, of which 1461 are new. The integration of all
the interactions reported in H. pylori results in 3004
unique interactions that connect about $70\%$ of its
proteome. Excluding interactions of promiscuous proteins we
derived from our new data a core network consisting of 908
interactions. We compared our data set to several other
bacterial interactomes and experimentally benchmarked the
conservation of interactions using 365 protein pairs
(interologs) of E. coli of which one third turned out to be
conserved in both species.},
keywords = {Bacterial Proteins (NLM Chemicals) / Proteome (NLM
Chemicals)},
cin = {W150 / W110},
ddc = {540},
cid = {I:(DE-He78)W150-20160331 / I:(DE-He78)W110-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:24627523},
pmc = {pmc:PMC4014287},
doi = {10.1074/mcp.O113.033571},
url = {https://inrepo02.dkfz.de/record/120175},
}