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000120363 037__ $$aDKFZ-2017-00797
000120363 041__ $$aeng
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000120363 1001_ $$aGéraud, Cyrill$$b0
000120363 245__ $$aGATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.
000120363 260__ $$aAnn Arbor, Mich.$$bASCJ$$c2017
000120363 3367_ $$2DRIVER$$aarticle
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000120363 520__ $$aMicrovascular endothelial cells (ECs) are increasingly recognized as organ-specific gatekeepers of their microenvironment. Microvascular ECs instruct neighboring cells in their organ-specific vascular niches through angiocrine factors, which include secreted growth factors (angiokines), extracellular matrix molecules, and transmembrane proteins. However, the molecular regulators that drive organ-specific microvascular transcriptional programs and thereby regulate angiodiversity are largely elusive. In contrast to other ECs, which form a continuous cell layer, liver sinusoidal ECs (LSECs) constitute discontinuous, permeable microvessels. Here, we have shown that the transcription factor GATA4 controls murine LSEC specification and function. LSEC-restricted deletion of Gata4 caused transformation of discontinuous liver sinusoids into continuous capillaries. Capillarization was characterized by ectopic basement membrane deposition, formation of a continuous EC layer, and increased expression of VE-cadherin. Correspondingly, ectopic expression of GATA4 in cultured continuous ECs mediated the downregulation of continuous EC-associated transcripts and upregulation of LSEC-associated genes. The switch from discontinuous LSECs to continuous ECs during embryogenesis caused liver hypoplasia, fibrosis, and impaired colonization by hematopoietic progenitor cells, resulting in anemia and embryonic lethality. Thus, GATA4 acts as master regulator of hepatic microvascular specification and acquisition of organ-specific vascular competence, which are indispensable for liver development. The data also establish an essential role of the hepatic microvasculature in embryonic hematopoiesis.
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000120363 7001_ $$aKoch, Philipp-Sebastian$$b1
000120363 7001_ $$aZierow, Johanna$$b2
000120363 7001_ $$0P:(DE-He78)8fc38a58dbca74c631b370f104fc2126$$aKlapproth, Kay$$b3
000120363 7001_ $$0P:(DE-He78)6695811f3270b3dbe3d06842e2ca55cd$$aBusch, Katrin$$b4
000120363 7001_ $$aOlsavszky, Victor$$b5
000120363 7001_ $$aLeibing, Thomas$$b6
000120363 7001_ $$aDemory, Alexandra$$b7
000120363 7001_ $$aUlbrich, Friederike$$b8
000120363 7001_ $$aDiett, Miriam$$b9
000120363 7001_ $$aSingh, Sandhya$$b10
000120363 7001_ $$aSticht, Carsten$$b11
000120363 7001_ $$aBreitkopf-Heinlein, Katja$$b12
000120363 7001_ $$0P:(DE-He78)027fe772631b4a2d7a45c439cdd75ff2$$aRichter, Karsten$$b13$$udkfz
000120363 7001_ $$aKarppinen, Sanna-Maria$$b14
000120363 7001_ $$aPihlajaniemi, Taina$$b15
000120363 7001_ $$0P:(DE-He78)554abc2af2666cc3421cc0d7f6e7b6de$$aArnold, Bernd$$b16$$udkfz
000120363 7001_ $$0P:(DE-He78)86fa3316b7be0d661065d02b3baec3d6$$aRodewald, Hans-Reimer$$b17
000120363 7001_ $$0P:(DE-He78)2e92d0ae281932fc7347d819fec36b0b$$aAugustin, Hellmut$$b18
000120363 7001_ $$aSchledzewski, Kai$$b19
000120363 7001_ $$aGoerdt, Sergij$$b20
000120363 773__ $$0PERI:(DE-600)2018375-6$$a10.1172/JCI90086$$gVol. 127, no. 3, p. 1099 - 1114$$n3$$p1099 - 1114$$tThe @journal of clinical investigation$$v127$$x1558-8238$$y2017
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