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@ARTICLE{Vlijm:120446,
      author       = {R. Vlijm$^*$ and S. H. Kim and P. L. De Zwart and Y. Dalal
                      and C. Dekker},
      title        = {{T}he supercoiling state of {DNA} determines the handedness
                      of both {H}3 and {CENP}-{A} nucleosomes.190},
      journal      = {Nanoscale},
      volume       = {9},
      number       = {5},
      issn         = {2040-3372},
      address      = {Cambridge},
      publisher    = {RSC Publ.},
      reportid     = {DKFZ-2017-00875},
      pages        = {1862 - 1870},
      year         = {2017},
      abstract     = {Nucleosomes form the unit structure of the genome in
                      eukaryotes, thereby constituting a fundamental tenet of
                      chromatin biology. In canonical nucleosomes, DNA wraps
                      around the histone octamer in a left-handed toroidal ramp.
                      Here, in single-molecule magnetic tweezers studies of
                      chaperone-assisted nucleosome assembly, we show that the
                      handedness of the DNA wrapping around the nucleosome core is
                      intrinsically ambidextrous, and depends on the pre-assembly
                      supercoiling state of the DNA, i.e., it is not uniquely
                      determined by the octameric histone core. Nucleosomes
                      assembled onto negatively supercoiled DNA are found to
                      exhibit a left-handed conformation, whereas assembly onto
                      positively supercoiled DNA results in right-handed
                      nucleosomes. This intrinsic flexibility to adopt both
                      chiralities is observed both for canonical H3 nucleosomes,
                      and for centromere-specific variant CENP-A nucleosomes.
                      These data support recent advances suggesting an intrinsic
                      adaptability of the nucleosome, and provide insights into
                      how nucleosomes might rapidly re-assemble after cellular
                      processes that generate positive supercoiling in vivo.},
      cin          = {E190},
      ddc          = {600},
      cid          = {I:(DE-He78)E190-20160331},
      pnm          = {315 - Imaging and radiooncology (POF3-315)},
      pid          = {G:(DE-HGF)POF3-315},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:28094382},
      doi          = {10.1039/C6NR06245H},
      url          = {https://inrepo02.dkfz.de/record/120446},
}