TY - JOUR AU - Morrissy, A Sorana AU - Cavalli, Florence M G AU - Remke, Marc AU - Ramaswamy, Vijay AU - Shih, David J H AU - Holgado, Borja L AU - Farooq, Hamza AU - Donovan, Laura K AU - Garzia, Livia AU - Agnihotri, Sameer AU - Kiehna, Erin N AU - Mercier, Eloi AU - Mayoh, Chelsea AU - Papillon-Cavanagh, Simon AU - Nikbakht, Hamid AU - Gayden, Tenzin AU - Torchia, Jonathon AU - Picard, Daniel AU - Merino, Diana M AU - Vladoiu, Maria AU - Luu, Betty AU - Wu, Xiaochong AU - Daniels, Craig AU - Horswell, Stuart AU - Thompson, Yuan Yao AU - Hovestadt, Volker AU - Northcott, Paul A AU - Jones, David AU - Peacock, John AU - Wang, Xin AU - Mack, Stephen C AU - Reimand, Jüri AU - Albrecht, Steffen AU - Fontebasso, Adam M AU - Thiessen, Nina AU - Li, Yisu AU - Schein, Jacqueline E AU - Lee, Darlene AU - Carlsen, Rebecca AU - Mayo, Michael AU - Tse, Kane AU - Tam, Angela AU - Dhalla, Noreen AU - Ally, Adrian AU - Chuah, Eric AU - Cheng, Young AU - Plettner, Patrick AU - Li, Haiyan I AU - Corbett, Richard D AU - Wong, Tina AU - Long, William AU - Loukides, James AU - Buczkowicz, Pawel AU - Hawkins, Cynthia E AU - Tabori, Uri AU - Rood, Brian R AU - Myseros, John S AU - Packer, Roger J AU - Korshunov, Andrey AU - Lichter, Peter AU - Kool, Marcel AU - Pfister, Stefan AU - Schüller, Ulrich AU - Dirks, Peter AU - Huang, Annie AU - Bouffet, Eric AU - Rutka, James T AU - Bader, Gary D AU - Swanton, Charles AU - Ma, Yusanne AU - Moore, Richard A AU - Mungall, Andrew J AU - Majewski, Jacek AU - Jones, Steven J M AU - Das, Sunit AU - Malkin, David AU - Jabado, Nada AU - Marra, Marco A AU - Taylor, Michael D TI - Spatial heterogeneity in medulloblastoma. JO - Nature genetics VL - 49 IS - 5 SN - 1546-1718 CY - New York, NY PB - Nature America M1 - DKFZ-2017-01079 SP - 780 - 788 PY - 2017 AB - Spatial heterogeneity of transcriptional and genetic markers between physically isolated biopsies of a single tumor poses major barriers to the identification of biomarkers and the development of targeted therapies that will be effective against the entire tumor. We analyzed the spatial heterogeneity of multiregional biopsies from 35 patients, using a combination of transcriptomic and genomic profiles. Medulloblastomas (MBs), but not high-grade gliomas (HGGs), demonstrated spatially homogeneous transcriptomes, which allowed for accurate subgrouping of tumors from a single biopsy. Conversely, somatic mutations that affect genes suitable for targeted therapeutics demonstrated high levels of spatial heterogeneity in MB, malignant glioma, and renal cell carcinoma (RCC). Actionable targets found in a single MB biopsy were seldom clonal across the entire tumor, which brings the efficacy of monotherapies against a single target into question. Clinical trials of targeted therapies for MB should first ensure the spatially ubiquitous nature of the target mutation. LB - PUB:(DE-HGF)16 C6 - pmid:28394352 DO - DOI:10.1038/ng.3838 UR - https://inrepo02.dkfz.de/record/120652 ER -