000120656 001__ 120656
000120656 005__ 20240228145459.0
000120656 0247_ $$2doi$$a10.1038/s41598-017-01258-6
000120656 0247_ $$2pmid$$apmid:28442745
000120656 0247_ $$2pmc$$apmc:PMC5430633
000120656 0247_ $$2altmetric$$aaltmetric:19627371
000120656 037__ $$aDKFZ-2017-01083
000120656 041__ $$aeng
000120656 082__ $$a000
000120656 1001_ $$00000-0002-2164-2323$$aRogov, Vladimir V$$b0
000120656 245__ $$aPhosphorylation of the mitochondrial autophagy receptor Nix enhances its interaction with LC3 proteins.
000120656 260__ $$aLondon$$bNature Publishing Group$$c2017
000120656 3367_ $$2DRIVER$$aarticle
000120656 3367_ $$2DataCite$$aOutput Types/Journal article
000120656 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1510837272_26037
000120656 3367_ $$2BibTeX$$aARTICLE
000120656 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000120656 3367_ $$00$$2EndNote$$aJournal Article
000120656 520__ $$aThe mitophagy receptor Nix interacts with LC3/GABARAP proteins, targeting mitochondria into autophagosomes for degradation. Here we present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Substitution of Lys51 to Ala in LC3B abrogates binding of a phosphomimetic Nix mutant. Functionally, serine 34/35 phosphorylation enhances autophagosome recruitment to mitochondria in HeLa cells. Together, this study provides cellular, biochemical and biophysical evidence that phosphorylation of the LIR domain of Nix enhances mitophagy receptor engagement.
000120656 536__ $$0G:(DE-HGF)POF3-312$$a312 - Functional and structural genomics (POF3-312)$$cPOF3-312$$fPOF III$$x0
000120656 588__ $$aDataset connected to CrossRef, PubMed,
000120656 7001_ $$aSuzuki, Hironori$$b1
000120656 7001_ $$aMarinković, Mija$$b2
000120656 7001_ $$0P:(DE-He78)929b2109a63146eb30e74f712ad74596$$aLang, Verena$$b3$$udkfz
000120656 7001_ $$aKato, Ryuichi$$b4
000120656 7001_ $$aKawasaki, Masato$$b5
000120656 7001_ $$aBuljubašić, Maja$$b6
000120656 7001_ $$aŠprung, Matilda$$b7
000120656 7001_ $$aRogova, Natalia$$b8
000120656 7001_ $$aWakatsuki, Soichi$$b9
000120656 7001_ $$0P:(DE-HGF)0$$aHamacher-Brady, Anne$$b10
000120656 7001_ $$aDötsch, Volker$$b11
000120656 7001_ $$00000-0001-8156-9511$$aDikic, Ivan$$b12
000120656 7001_ $$0P:(DE-He78)5bf984e94f0a31773a103cd293e01f92$$aBrady, Nathan$$b13$$udkfz
000120656 7001_ $$aNovak, Ivana$$b14
000120656 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-017-01258-6$$gVol. 7, no. 1, p. 1131$$n1$$p1131$$tScientific reports$$v7$$x2045-2322$$y2017
000120656 909CO $$ooai:inrepo02.dkfz.de:120656$$pVDB
000120656 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)929b2109a63146eb30e74f712ad74596$$aDeutsches Krebsforschungszentrum$$b3$$kDKFZ
000120656 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-HGF)0$$aDeutsches Krebsforschungszentrum$$b10$$kDKFZ
000120656 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)5bf984e94f0a31773a103cd293e01f92$$aDeutsches Krebsforschungszentrum$$b13$$kDKFZ
000120656 9131_ $$0G:(DE-HGF)POF3-312$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vFunctional and structural genomics$$x0
000120656 9141_ $$y2017
000120656 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bSCI REP-UK : 2015
000120656 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000120656 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000120656 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000120656 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000120656 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000120656 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ
000120656 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000120656 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000120656 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000120656 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000120656 915__ $$0StatID:(DE-HGF)1150$$2StatID$$aDBCoverage$$bCurrent Contents - Physical, Chemical and Earth Sciences
000120656 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record
000120656 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000120656 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bSCI REP-UK : 2015
000120656 9201_ $$0I:(DE-He78)B190-20160331$$kB190$$le:Bio Nachwuchsgruppe Lysosomale Systembiologie$$x0
000120656 980__ $$ajournal
000120656 980__ $$aVDB
000120656 980__ $$aI:(DE-He78)B190-20160331
000120656 980__ $$aUNRESTRICTED