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@ARTICLE{Schepkin:120657,
author = {V. D. Schepkin and A. Neubauer and A. Nagel$^*$ and T. F.
Budinger},
title = {{C}omparison of potassium and sodium binding in vivo and in
agarose samples using {TQTPPI} pulse sequence.},
journal = {Journal of magnetic resonance},
volume = {277},
issn = {1090-7807},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {DKFZ-2017-01084},
pages = {162 - 168},
year = {2017},
abstract = {Potassium and sodium specific binding in vivo were explored
at 21.1T by triple quantum (TQ) magnetic resonance (MR)
signals without filtration to achieve high sensitivities and
precise quantifications. The pulse sequence used time
proportional phase increments (TPPI). During simultaneous
phase-time increments, it provided total single quantum (SQ)
and TQ MR signals in the second dimension at single and
triple quantum frequencies, respectively. The detection of
both TQ and SQ signals was performed at identical
experimental conditions and the resulting TQ signal equals
$60±3\%$ of the SQ signal when all ions experience
sufficient time for binding. In a rat head in vivo the TQ
percentage relative to SQ for potassium is $41.5±3\%$ and
for sodium is $16.1±1\%.$ These percentages were compared
to the matching values in an agarose tissue model with MR
relaxation times similar to those of mammalian brain tissue.
The sodium TQ signal in agarose samples decreased in the
presence of potassium, suggesting a competitive binding of
potassium relative to sodium ions for the same binding
sites. The TQTPPI signals correspond to almost two times
more effective binding of potassium than sodium. In vivo, up
to $∼69\%$ of total potassium and $∼27\%$ of total
sodium can be regarded as bound or experiencing an
association time in the range of several milliseconds.
Experimental data analyses show that more than half of the
in vivo total sodium TQ signal could be from extracellular
space, which is an important factor for quantification of
intracellular MR signals.},
cin = {E020},
ddc = {550},
cid = {I:(DE-He78)E020-20160331},
pnm = {315 - Imaging and radiooncology (POF3-315)},
pid = {G:(DE-HGF)POF3-315},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28314206},
doi = {10.1016/j.jmr.2017.03.003},
url = {https://inrepo02.dkfz.de/record/120657},
}