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@ARTICLE{Bohlnder:125535,
      author       = {P. R. Bohländer and M. L. Abba and F. Bestvater$^*$ and H.
                      Allgayer$^*$ and H.-A. Wagenknecht},
      title        = {{T}wo wavelength-shifting molecular beacons for
                      simultaneous and selective imaging of vesicular mi{RNA}-21
                      and mi{RNA}-31 in living cancer cells.},
      journal      = {Organic $\&$ biomolecular chemistry},
      volume       = {14},
      number       = {22},
      issn         = {1477-0539},
      address      = {Cambridge},
      publisher    = {Royal Society of Chemistry},
      reportid     = {DKFZ-2017-01661},
      pages        = {5001 - 5006},
      year         = {2016},
      abstract     = {Two molecular beacons were designed as complementary
                      fluorescent imaging probes for miRNA-21 and miRNA-31. Both
                      beacons were prepared by a combination of solid-phase
                      protocol and Cu(i)-catalyzed cycloaddition chemistry. The
                      four photostable and bright fluorophores were attached to
                      2'-positions in the stem part of the two beacons. One beacon
                      was labeled by a green-to-red emitting and the other by a
                      blue-to-yellow emitting energy transfer pair. This two by
                      two combination yields the four color emission readout. In
                      vitro experiments demonstrate rapid and highly selective
                      opening of both molecular beacons upon addition of the
                      complementary target RNA and excellent green : red and
                      blue : yellow emission color contrasts. Confocal
                      microscopy of selected cancer cell lines provides evidence
                      that a four color imaging of versicular miRNA-21 and
                      miRNA-31 can be achieved both selectively and simultaneously
                      upon transfection by the beacons, and that the fluorescent
                      readouts track well with miRNA levels determined by PCR.},
      cin          = {G360 / W210},
      ddc          = {540},
      cid          = {I:(DE-He78)G360-20160331 / I:(DE-He78)W210-20160331},
      pnm          = {317 - Translational cancer research (POF3-317)},
      pid          = {G:(DE-HGF)POF3-317},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27114268},
      doi          = {10.1039/C6OB00691D},
      url          = {https://inrepo02.dkfz.de/record/125535},
}