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@ARTICLE{Aschauer:126063,
      author       = {L. Aschauer and A. Limonciel and A. Wilmes and S.
                      Stanzel$^*$ and A. Kopp-Schneider$^*$ and P. Hewitt and A.
                      Lukas and M. O. Leonard and W. Pfaller and P. Jennings},
      title        = {{A}pplication of {RPTEC}/{TERT}1 cells for investigation of
                      repeat dose nephrotoxicity: {A} transcriptomic study.},
      journal      = {Toxicology in vitro},
      volume       = {30},
      number       = {1 Pt A},
      issn         = {0887-2333},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DKFZ-2017-02178},
      pages        = {106 - 116},
      year         = {2015},
      abstract     = {The kidney is a major target organ for toxicity. Incidence
                      of chronic kidney disease (CKD) is increasing at an alarming
                      rate due to factors such as increasing population age and
                      increased prevalence of heart disease and diabetes. There is
                      a major effort ongoing to develop superior predictive models
                      of renal injury and early renal biomarkers that can predict
                      onset of CKD. In the EU FP7 funded project, Predict-IV, we
                      investigated the human renal proximal tubule cells line,
                      RPTEC/TERT1 for their applicability to long term nephrotoxic
                      mechanistic studies. To this end, we used a tiered strategy
                      to optimise dosing regimes for 9 nephrotoxins. Our final
                      testing protocol utilised differentiated RPTEC/TERT1 cells
                      cultured on filter inserts treated with compounds at both
                      the apical and basolateral side, at concentrations not
                      exceeding IC10, for 14 days in a 24 h repeat application.
                      Transepithelial electrical resistance and supernatant
                      lactate were measured over the duration of the experiments
                      and genome wide transcriptomic profiles were assayed at day
                      1, 3 and 14. The effect of hypoxia was investigated for a
                      subset of compounds. The transcriptomic data were analysed
                      to investigate compound-specific effects, global responses
                      and mechanistically informative signatures. In addition,
                      several potential clinically useful renal injury biomarkers
                      were identified.},
      keywords     = {Lactates (NLM Chemicals) / Pharmaceutical Preparations (NLM
                      Chemicals)},
      cin          = {C060},
      ddc          = {610},
      cid          = {I:(DE-He78)C060-20160331},
      pnm          = {313 - Cancer risk factors and prevention (POF3-313)},
      pid          = {G:(DE-HGF)POF3-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:25450743},
      doi          = {10.1016/j.tiv.2014.10.005},
      url          = {https://inrepo02.dkfz.de/record/126063},
}