TY  - JOUR
AU  - Bhatia, Shilpa
AU  - Baig, Nimrah A
AU  - Timofeeva, Olga
AU  - Pasquale, Elena B
AU  - Hirsch, Kellen
AU  - MacDonald, Tobey J
AU  - Dritschilo, Anatoly
AU  - Lee, Yi Chien
AU  - Henkemeyer, Mark
AU  - Rood, Brian
AU  - Jung, Mira
AU  - Wang, Xiao-Jing
AU  - Kool, Marcel
AU  - Rodriguez, Olga
AU  - Albanese, Chris
AU  - Karam, Sana D
TI  - Knockdown of EphB1 receptor decreases medulloblastoma cell growth and migration and increases cellular radiosensitization.
JO  - OncoTarget
VL  - 6
IS  - 11
SN  - 1949-2553
CY  - [S.l.]
PB  - Impact Journals LLC
M1  - DKFZ-2017-02259
SP  - 8929 - 8946
PY  - 2015
AB  - The expression of members of the Eph family of receptor tyrosine kinases and their ephrin ligands is frequently dysregulated in medulloblastomas. We assessed the expression and functional role of EphB1 in medulloblastoma cell lines and engineered mouse models. mRNA and protein expression profiling showed expression of EphB1 receptor in the human medulloblastoma cell lines DAOY and UW228. EphB1 downregulation reduced cell growth and viability, decreased the expression of important cell cycle regulators, and increased the percentage of cells in G1 phase of the cell cycle. It also modulated the expression of proliferation, and cell survival markers. In addition, EphB1 knockdown in DAOY cells resulted in significant decrease in migration, which correlated with decreased β1-integrin expression and levels of phosphorylated Src. Furthermore, EphB1 knockdown enhanced cellular radiosensitization of medulloblastoma cells in culture and in a genetically engineered mouse medulloblastoma model. Using genetically engineered mouse models, we established that genetic loss of EphB1 resulted in a significant delay in tumor recurrence following irradiation compared to EphB1-expressing control tumors. Taken together, our findings establish that EphB1 plays a key role in medulloblastoma cell growth, viability, migration, and radiation sensitivity, making EphB1 a promising therapeutic target.
KW  - Antigens, CD29 (NLM Chemicals)
KW  - Cell Cycle Proteins (NLM Chemicals)
KW  - Neoplasm Proteins (NLM Chemicals)
KW  - RNA, Small Interfering (NLM Chemicals)
KW  - Receptor, EphB1 (NLM Chemicals)
KW  - Proto-Oncogene Proteins pp60(c-src) (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:25879388
C2  - pmc:PMC4496193
DO  - DOI:10.18632/oncotarget.3369
UR  - https://inrepo02.dkfz.de/record/126144
ER  -