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@ARTICLE{Blume:126160,
author = {C. J. Blume$^*$ and A. Hotz-Wagenblatt$^*$ and J.
Hüllein$^*$ and L. Sellner$^*$ and A. Jethwa$^*$ and T.
Stolz$^*$ and M. Slabicki$^*$ and K. Lee$^*$ and A.
Sharathchandra and A. Benner$^*$ and S. Dietrich$^*$ and C.
C. Oakes$^*$ and P. Dreger and D. te Raa and A. P. Kater and
A. Jauch and O. Merkel$^*$ and M. Oren and T. Hielscher$^*$
and T. Zenz$^*$},
title = {p53-dependent non-coding {RNA} networks in chronic
lymphocytic leukemia.},
journal = {Leukemia},
volume = {29},
number = {10},
issn = {1476-5551},
address = {Basingstoke},
publisher = {Nature Publ. Group},
reportid = {DKFZ-2017-02275},
pages = {2015 - 2023},
year = {2015},
abstract = {Mutations of the tumor suppressor p53 lead to chemotherapy
resistance and a dismal prognosis in chronic lymphocytic
leukemia (CLL). Whereas p53 targets are used to identify
patient subgroups with impaired p53 function, a
comprehensive assessment of non-coding RNA targets of p53 in
CLL is missing. We exploited the impaired transcriptional
activity of mutant p53 to map out p53 targets in CLL by
small RNA sequencing. We describe the landscape of
p53-dependent microRNA/non-coding RNA induced in response to
DNA damage in CLL. Besides the key p53 target miR-34a, we
identify a set of p53-dependent microRNAs (miRNAs;
miR-182-5p, miR-7-5p and miR-320c/d). In addition to miRNAs,
the long non-coding RNAs (lncRNAs) nuclear enriched abundant
transcript 1 (NEAT1) and long intergenic non-coding RNA p21
(lincRNA-p21) are induced in response to DNA damage in the
presence of functional p53 but not in CLL with p53 mutation.
Induction of NEAT1 and lincRNA-p21 are closely correlated to
the induction of cell death after DNA damage. We used
isogenic lymphoma cell line models to prove p53 dependence
of NEAT1 and lincRNA-p21. The current work describes the
p53-dependent miRNome and identifies lncRNAs NEAT1 and
lincRNA-p21 as novel elements of the p53-dependent DNA
damage response machinery in CLL and lymphoma.},
keywords = {MicroRNAs (NLM Chemicals) / NEAT1 long non-coding RNA,
human (NLM Chemicals) / RNA, Long Noncoding (NLM Chemicals)
/ RNA, Messenger (NLM Chemicals) / TP53 protein, human (NLM
Chemicals) / Tumor Suppressor Protein p53 (NLM Chemicals)},
cin = {G100 / W180 / C060},
ddc = {610},
cid = {I:(DE-He78)G100-20160331 / I:(DE-He78)W180-20160331 /
I:(DE-He78)C060-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:25971364},
doi = {10.1038/leu.2015.119},
url = {https://inrepo02.dkfz.de/record/126160},
}