%0 Journal Article
%A Busch, Katrin
%A Klapproth, Kay
%A Barile, Melania
%A Flossdorf, Michael
%A Holland-Letz, Tim
%A Schlenner, Susan M
%A Reth, Michael
%A Höfer, Thomas
%A Rodewald, Hans-Reimer
%T Fundamental properties of unperturbed haematopoiesis from stem cells in vivo.
%J Nature 
%V 518
%N 7540
%@ 1476-4687
%C London [u.a.]
%I Nature Publ. Group
%M DKFZ-2017-02330
%P 542 - 546
%D 2015
%X Haematopoietic stem cells (HSCs) are widely studied by HSC transplantation into immune- and blood-cell-depleted recipients. Single HSCs can rebuild the system after transplantation. Chromosomal marking, viral integration and barcoding of transplanted HSCs suggest that very low numbers of HSCs perpetuate a continuous stream of differentiating cells. However, the numbers of productive HSCs during normal haematopoiesis, and the flux of differentiating progeny remain unknown. Here we devise a mouse model allowing inducible genetic labelling of the most primitive Tie2(+) HSCs in bone marrow, and quantify label progression along haematopoietic development by limiting dilution analysis and data-driven modelling. During maintenance of the haematopoietic system, at least 30
%K Receptor, TIE-2 (NLM Chemicals)
%K Tek protein, mouse (NLM Chemicals)
%K Fluorouracil (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:25686605
%R 10.1038/nature14242
%U https://inrepo02.dkfz.de/record/126215