TY  - JOUR
AU  - Busch, Katrin
AU  - Klapproth, Kay
AU  - Barile, Melania
AU  - Flossdorf, Michael
AU  - Holland-Letz, Tim
AU  - Schlenner, Susan M
AU  - Reth, Michael
AU  - Höfer, Thomas
AU  - Rodewald, Hans-Reimer
TI  - Fundamental properties of unperturbed haematopoiesis from stem cells in vivo.
JO  - Nature 
VL  - 518
IS  - 7540
SN  - 1476-4687
CY  - London [u.a.]
PB  - Nature Publ. Group
M1  - DKFZ-2017-02330
SP  - 542 - 546
PY  - 2015
AB  - Haematopoietic stem cells (HSCs) are widely studied by HSC transplantation into immune- and blood-cell-depleted recipients. Single HSCs can rebuild the system after transplantation. Chromosomal marking, viral integration and barcoding of transplanted HSCs suggest that very low numbers of HSCs perpetuate a continuous stream of differentiating cells. However, the numbers of productive HSCs during normal haematopoiesis, and the flux of differentiating progeny remain unknown. Here we devise a mouse model allowing inducible genetic labelling of the most primitive Tie2(+) HSCs in bone marrow, and quantify label progression along haematopoietic development by limiting dilution analysis and data-driven modelling. During maintenance of the haematopoietic system, at least 30
KW  - Receptor, TIE-2 (NLM Chemicals)
KW  - Tek protein, mouse (NLM Chemicals)
KW  - Fluorouracil (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:25686605
DO  - DOI:10.1038/nature14242
UR  - https://inrepo02.dkfz.de/record/126215
ER  -