%0 Journal Article
%A Goschzik, Tobias
%A Zur Mühlen, Anja
%A Kristiansen, Glen
%A Haberler, Christine
%A Stefanits, Harald
%A Friedrich, Carsten
%A von Hoff, Katja
%A Rutkowski, Stefan
%A Pfister, Stefan
%A Pietsch, Torsten
%T Molecular stratification of medulloblastoma: comparison of histological and genetic methods to detect Wnt activated tumours.
%J Neuropathology & applied neurobiology
%V 41
%N 2
%@ 0305-1846
%C Oxford [u.a.]
%I Wiley-Blackwell
%M DKFZ-2017-02637
%P 135 - 144
%D 2015
%X Wnt activation in medulloblastomas is associated with good outcome. Upfront testing and risk-adapted stratification of patients will be done in future clinical studies. In a cohort of 186 paediatric medulloblastomas our aim was to identify the optimal methods in standard clinical practice to detect this subgroup.Nuclear accumulation of β-catenin was analysed by immunohistochemistry (IHC). DNA of FFPE tissue was amplified by PCR for single-strand conformation polymorphism analysis and direct sequencing of CTNNB1 exon 3. Copy number of chromosome 6 was analysed by multiplex ligation-dependent probe amplification and molecular inversion profiling.Different automated immunostaining systems showed similar results. Twenty-one of 186 samples had nuclear accumulation in ≥5
%K CTNNB1 protein, human (NLM Chemicals)
%K beta Catenin (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:24894640
%R 10.1111/nan.12161
%U https://inrepo02.dkfz.de/record/126609