% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Haller:126653,
      author       = {F. Haller and J. D. Zhang$^*$ and E. A. Moskalev and A.
                      Braun and C. Otto and H. Geddert and Y. Riazalhosseini$^*$
                      and A. Ward$^*$ and A. Balwierz$^*$ and I.-M. Schaefer and
                      S. Cameron and B. M. Ghadimi and A. Agaimy and J. A.
                      Fletcher and J. Hoheisel$^*$ and A. Hartmann and M. Werner
                      and S. Wiemann$^*$ and O. Sahin$^*$},
      title        = {{C}ombined {DNA} methylation and gene expression profiling
                      in gastrointestinal stromal tumors reveals hypomethylation
                      of {SPP}1 as an independent prognostic factor.},
      journal      = {International journal of cancer},
      volume       = {136},
      number       = {5},
      issn         = {0020-7136},
      address      = {Bognor Regis},
      publisher    = {Wiley-Liss},
      reportid     = {DKFZ-2017-02681},
      pages        = {1013 - 1023},
      year         = {2015},
      abstract     = {Gastrointestinal stromal tumors (GISTs) have distinct gene
                      expression patterns according to localization, genotype and
                      aggressiveness. DNA methylation at CpG dinucleotides is an
                      important mechanism for regulation of gene expression. We
                      performed targeted DNA methylation analysis of 1.505 CpG
                      loci in 807 cancer-related genes in a cohort of 76 GISTs,
                      combined with genome-wide mRNA expression analysis in 22
                      GISTs, to identify signatures associated with
                      clinicopathological parameters and prognosis. Principal
                      component analysis revealed distinct DNA methylation
                      patterns associated with anatomical localization, genotype,
                      mitotic counts and clinical follow-up. Methylation of a
                      single CpG dinucleotide in the non-CpG island promoter of
                      SPP1 was significantly correlated with shorter disease-free
                      survival. Hypomethylation of this CpG was an independent
                      prognostic parameter in a multivariate analysis compared to
                      anatomical localization, genotype, tumor size and mitotic
                      counts in a cohort of 141 GISTs with clinical follow-up. The
                      epigenetic regulation of SPP1 was confirmed in vitro, and
                      the functional impact of SPP1 protein on
                      tumorigenesis-related signaling pathways was demonstrated.
                      In summary, SPP1 promoter methylation is a novel and
                      independent prognostic parameter in GISTs, and might be
                      helpful in estimating the aggressiveness of GISTs from the
                      intermediate-risk category.},
      keywords     = {Biomarkers, Tumor (NLM Chemicals) / SPP1 protein, human
                      (NLM Chemicals) / Osteopontin (NLM Chemicals)},
      cin          = {B050 / B070 / W110},
      ddc          = {610},
      cid          = {I:(DE-He78)B050-20160331 / I:(DE-He78)B070-20160331 /
                      I:(DE-He78)W110-20160331},
      pnm          = {312 - Functional and structural genomics (POF3-312)},
      pid          = {G:(DE-HGF)POF3-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:25046773},
      doi          = {10.1002/ijc.29088},
      url          = {https://inrepo02.dkfz.de/record/126653},
}