TY  - JOUR
AU  - Hemminki, Otto
AU  - Parviainen, Suvi
AU  - Juhila, Juuso
AU  - Turkki, Riku
AU  - Linder, Nina
AU  - Lundin, Johan
AU  - Kankainen, Matti
AU  - Ristimäki, Ari
AU  - Koski, Anniina
AU  - Liikanen, Ilkka
AU  - Oksanen, Minna
AU  - Nettelbeck, Dirk
AU  - Kairemo, Kalevi
AU  - Partanen, Kaarina
AU  - Joensuu, Timo
AU  - Kanerva, Anna
AU  - Hemminki, Akseli
TI  - Immunological data from cancer patients treated with Ad5/3-E2F-Δ24-GMCSF suggests utility for tumor immunotherapy.
JO  - OncoTarget
VL  - 6
IS  - 6
SN  - 1949-2553
CY  - [S.l.]
PB  - Impact Journals LLC
M1  - DKFZ-2017-02734
SP  - 4467 - 4481
PY  - 2015
AB  - Oncolytic viruses that selectively replicate in tumor cells can be used for treatment of cancer. Accumulating data suggests that virus induced oncolysis can enhance anti-tumor immunity and break immune tolerance. To capitalize on the immunogenic nature of oncolysis, we generated a quadruple modified oncolytic adenovirus expressing granulocyte-macrophage colony-stimulating factor (GMCSF). Ad5/3-E2F-Δ24-GMCSF (CGTG-602) was engineered to contain a tumor specific E2F1 promoter driving an E1 gene deleted at the retinoblastoma protein binding site ('Δ24'). The fiber features a knob from serotype 3 for enhanced gene delivery to tumor cells. The virus was tested preclinically in vitro and in vivo and then 13 patients with solid tumors refractory to standard therapies were treated. Treatments were well tolerated and frequent tumor- and adenovirus-specific T-cell immune responses were seen. Overall, with regard to tumor marker or radiological responses, signs of antitumor efficacy were seen in 9/12 evaluable patients (75
KW  - Antibodies, Neutralizing (NLM Chemicals)
KW  - Antibodies, Viral (NLM Chemicals)
KW  - E2F1 Transcription Factor (NLM Chemicals)
KW  - E2F1 protein, human (NLM Chemicals)
KW  - Granulocyte-Macrophage Colony-Stimulating Factor (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:25714011
C2  - pmc:PMC4414204
DO  - DOI:10.18632/oncotarget.2901
UR  - https://inrepo02.dkfz.de/record/126706
ER  -