%0 Journal Article
%A Kiesow, Kristin
%A Bennewitz, Katrin
%A Miranda, Laura Gutierrez
%A Stoll, Sandra J
%A Hartenstein, Bettina
%A Angel, Peter
%A Kroll, Jens
%A Schorpp-Kistner, Marina
%T Junb controls lymphatic vascular development in zebrafish via miR-182.
%J Scientific reports
%V 5
%@ 2045-2322
%C London
%I Nature Publishing Group
%M DKFZ-2017-02901
%P 15007 -
%D 2015
%X JUNB, a subunit of the AP-1 transcription factor complex, mediates gene regulation in response to a plethora of extracellular stimuli. Previously, JUNB was shown to act as a critical positive regulator of blood vessel development and homeostasis as well as a negative regulator of proliferation, inflammation and tumour growth. Here, we demonstrate that the oncogenic miR-182 is a novel JUNB target. Loss-of-function studies by morpholino-mediated knockdown and the CRISPR/Cas9 technology identify a novel function for both JUNB and its target miR-182 in lymphatic vascular development in zebrafish. Furthermore, we show that miR-182 attenuates foxo1 expression indicating that strictly balanced Foxo1 levels are required for proper lymphatic vascular development in zebrafish. In conclusion, our findings uncover with the Junb/miR-182/Foxo1 regulatory axis a novel key player in governing lymphatic vascular morphogenesis in zebrafish.
%K Forkhead Box Protein O1 (NLM Chemicals)
%K Forkhead Transcription Factors (NLM Chemicals)
%K FoxO1 protein, zebrafish (NLM Chemicals)
%K MicroRNAs (NLM Chemicals)
%K Proto-Oncogene Proteins c-jun (NLM Chemicals)
%K Zebrafish Proteins (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:26458334
%2 pmc:PMC4602192
%R 10.1038/srep15007
%U https://inrepo02.dkfz.de/record/126873