TY  - JOUR
AU  - Kiesow, Kristin
AU  - Bennewitz, Katrin
AU  - Miranda, Laura Gutierrez
AU  - Stoll, Sandra J
AU  - Hartenstein, Bettina
AU  - Angel, Peter
AU  - Kroll, Jens
AU  - Schorpp-Kistner, Marina
TI  - Junb controls lymphatic vascular development in zebrafish via miR-182.
JO  - Scientific reports
VL  - 5
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - DKFZ-2017-02901
SP  - 15007 -
PY  - 2015
AB  - JUNB, a subunit of the AP-1 transcription factor complex, mediates gene regulation in response to a plethora of extracellular stimuli. Previously, JUNB was shown to act as a critical positive regulator of blood vessel development and homeostasis as well as a negative regulator of proliferation, inflammation and tumour growth. Here, we demonstrate that the oncogenic miR-182 is a novel JUNB target. Loss-of-function studies by morpholino-mediated knockdown and the CRISPR/Cas9 technology identify a novel function for both JUNB and its target miR-182 in lymphatic vascular development in zebrafish. Furthermore, we show that miR-182 attenuates foxo1 expression indicating that strictly balanced Foxo1 levels are required for proper lymphatic vascular development in zebrafish. In conclusion, our findings uncover with the Junb/miR-182/Foxo1 regulatory axis a novel key player in governing lymphatic vascular morphogenesis in zebrafish.
KW  - Forkhead Box Protein O1 (NLM Chemicals)
KW  - Forkhead Transcription Factors (NLM Chemicals)
KW  - FoxO1 protein, zebrafish (NLM Chemicals)
KW  - MicroRNAs (NLM Chemicals)
KW  - Proto-Oncogene Proteins c-jun (NLM Chemicals)
KW  - Zebrafish Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:26458334
C2  - pmc:PMC4602192
DO  - DOI:10.1038/srep15007
UR  - https://inrepo02.dkfz.de/record/126873
ER  -