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@ARTICLE{Limonciel:127014,
author = {A. Limonciel and K. Moenks and S. Stanzel and G. L. Truisi
and C. Parmentier and L. Aschauer and A. Wilmes and L.
Richert and P. Hewitt and S. O. Mueller and A. Lukas and A.
Kopp-Schneider$^*$ and M. O. Leonard and P. Jennings},
title = {{T}ranscriptomics hit the target: {M}onitoring of
ligand-activated and stress response pathways for chemical
testing.},
journal = {Toxicology in vitro},
volume = {30},
number = {1 Pt A},
issn = {0887-2333},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {DKFZ-2017-03040},
pages = {7 - 18},
year = {2015},
abstract = {High content omic methods provide a deep insight into
cellular events occurring upon chemical exposure of a cell
population or tissue. However, this improvement in analytic
precision is not yet matched by a thorough understanding of
molecular mechanisms that would allow an optimal
interpretation of these biological changes. For
transcriptomics (TCX), one type of molecular effects that
can be assessed already is the modulation of the
transcriptional activity of a transcription factor (TF). As
more ChIP-seq datasets reporting genes specifically bound by
a TF become publicly available for mining, the generation of
target gene lists of TFs of toxicological relevance becomes
possible, based on actual protein-DNA interaction and
modulation of gene expression. In this study, we generated
target gene signatures for Nrf2, ATF4, XBP1, p53, HIF1a, AhR
and PPAR gamma and tracked TF modulation in a large
collection of in vitro TCX datasets from renal and hepatic
cell models exposed to clinical nephro- and hepato-toxins.
The result is a global monitoring of TF modulation with
great promise as a mechanistically based tool for chemical
hazard identification.},
keywords = {Hazardous Substances (NLM Chemicals) / Ligands (NLM
Chemicals) / Transcription Factors (NLM Chemicals)},
cin = {C060},
ddc = {610},
cid = {I:(DE-He78)C060-20160331},
pnm = {313 - Cancer risk factors and prevention (POF3-313)},
pid = {G:(DE-HGF)POF3-313},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:25596134},
doi = {10.1016/j.tiv.2014.12.011},
url = {https://inrepo02.dkfz.de/record/127014},
}