TY  - JOUR
AU  - Koelsche, Christian
AU  - Schweizer, Leonille
AU  - Renner, Marcus
AU  - Warth, Arne
AU  - Jones, David
AU  - Sahm, Felix
AU  - Reuss, David E
AU  - Capper, David
AU  - Knösel, Thomas
AU  - Schulz, Birte
AU  - Petersen, Iver
AU  - Ulrich, Alexis
AU  - Renker, Eva Kristin
AU  - Lehner, Burkhard
AU  - Pfister, Stefan
AU  - Schirmacher, Peter
AU  - von Deimling, Andreas
AU  - Mechtersheimer, Gunhild
TI  - Nuclear relocation of STAT6 reliably predicts NAB2-STAT6 fusion for the diagnosis of solitary fibrous tumour.
JO  - Histopathology
VL  - 65
IS  - 5
SN  - 0309-0167
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - DKFZ-2017-03175
SP  - 613 - 622
PY  - 2014
AB  - Nuclear relocation of STAT6 has been shown in tumours with NAB2-STAT6 fusion, and has been proposed as an ancillary marker for the diagnosis of solitary fibrous tumours (SFTs). The aim of this study was to verify the utility of STAT6 immunohistology in diagnosing SFT.A total of 689 formalin-fixed paraffin-embedded tumours comprising 35 pleural SFTs and 654 other mesenchymal tumours were investigated for STAT6 expression using immunohistochemistry. Nine dedifferentiated liposarcomas (DDLSs) and five SFTs were also examined for the presence of NAB2-STAT6 fusion at the protein level using the proximity ligation assay (PLA), and for copy number variants (CNVs) with the Illumina Infinium Human Methylation450 array. Thirty-four of 35 SFTs showed strong nuclear STAT6 expression. Furthermore, five of 68 DDLSs, two of 130 undifferentiated pleomorphic sarcomas and one of 63 cases of nodular fasciitis showed moderate to strong nuclear STAT6 expression. The PLA indicated the presence of NAB2-STAT6 fusion protein in SFTs, but signal was also detected in some DDLSs. Copy number analysis showed an overall low frequency of chromosomal imbalances in SFTs, but complex karyotypes in DDLSs, including amplification of STAT6 and MDM2 loci.The detection of nuclear relocation of STAT6 with immunohistochemistry is a characteristic of SFTs, and may serve as a diagnostic marker that indicates NAB2-STAT6 fusion and helps to discriminate SFTs from histological mimics.
KW  - Biomarkers, Tumor (NLM Chemicals)
KW  - NAB2 protein, human (NLM Chemicals)
KW  - Repressor Proteins (NLM Chemicals)
KW  - STAT6 Transcription Factor (NLM Chemicals)
KW  - STAT6 protein, human (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:24702701
DO  - DOI:10.1111/his.12431
UR  - https://inrepo02.dkfz.de/record/127149
ER  -