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@ARTICLE{Koelsche:127149,
author = {C. Koelsche$^*$ and L. Schweizer$^*$ and M. Renner and A.
Warth and D. Jones$^*$ and F. Sahm$^*$ and D. E. Reuss$^*$
and D. Capper$^*$ and T. Knösel and B. Schulz and I.
Petersen and A. Ulrich and E. K. Renker and B. Lehner and S.
Pfister$^*$ and P. Schirmacher and A. von Deimling$^*$ and
G. Mechtersheimer},
title = {{N}uclear relocation of {STAT}6 reliably predicts
{NAB}2-{STAT}6 fusion for the diagnosis of solitary fibrous
tumour.},
journal = {Histopathology},
volume = {65},
number = {5},
issn = {0309-0167},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {DKFZ-2017-03175},
pages = {613 - 622},
year = {2014},
abstract = {Nuclear relocation of STAT6 has been shown in tumours with
NAB2-STAT6 fusion, and has been proposed as an ancillary
marker for the diagnosis of solitary fibrous tumours (SFTs).
The aim of this study was to verify the utility of STAT6
immunohistology in diagnosing SFT.A total of 689
formalin-fixed paraffin-embedded tumours comprising 35
pleural SFTs and 654 other mesenchymal tumours were
investigated for STAT6 expression using
immunohistochemistry. Nine dedifferentiated liposarcomas
(DDLSs) and five SFTs were also examined for the presence of
NAB2-STAT6 fusion at the protein level using the proximity
ligation assay (PLA), and for copy number variants (CNVs)
with the Illumina Infinium Human Methylation450 array.
Thirty-four of 35 SFTs showed strong nuclear STAT6
expression. Furthermore, five of 68 DDLSs, two of 130
undifferentiated pleomorphic sarcomas and one of 63 cases of
nodular fasciitis showed moderate to strong nuclear STAT6
expression. The PLA indicated the presence of NAB2-STAT6
fusion protein in SFTs, but signal was also detected in some
DDLSs. Copy number analysis showed an overall low frequency
of chromosomal imbalances in SFTs, but complex karyotypes in
DDLSs, including amplification of STAT6 and MDM2 loci.The
detection of nuclear relocation of STAT6 with
immunohistochemistry is a characteristic of SFTs, and may
serve as a diagnostic marker that indicates NAB2-STAT6
fusion and helps to discriminate SFTs from histological
mimics.},
keywords = {Biomarkers, Tumor (NLM Chemicals) / NAB2 protein, human
(NLM Chemicals) / Repressor Proteins (NLM Chemicals) / STAT6
Transcription Factor (NLM Chemicals) / STAT6 protein, human
(NLM Chemicals)},
cin = {G380 / B062},
ddc = {610},
cid = {I:(DE-He78)G380-20160331 / I:(DE-He78)B062-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:24702701},
doi = {10.1111/his.12431},
url = {https://inrepo02.dkfz.de/record/127149},
}