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@ARTICLE{Mudduluru:127177,
author = {G. Mudduluru$^*$ and M. L. Abba$^*$ and J. Batliner$^*$ and
N. S. Patil$^*$ and M. Scharp$^*$ and T. R. Lunavat$^*$ and
J. H. Leupold$^*$ and O. Oleksiuk$^*$ and D. Juraeva$^*$ and
W. Thiele and M. Rothley and A. Benner$^*$ and Y. Ben-Neriah
and J. Sleeman and H. Allgayer$^*$},
title = {{A} {S}ystematic {A}pproach to {D}efining the micro{RNA}
{L}andscape in {M}etastasis.},
journal = {Cancer research},
volume = {75},
number = {15},
issn = {1538-7445},
address = {Philadelphia, Pa.},
publisher = {AACR},
reportid = {DKFZ-2017-03203},
pages = {3010 - 3019},
year = {2015},
abstract = {The microRNA (miRNA) landscape changes during the
progression of cancer. We defined a metastasis-associated
miRNA landscape using a systematic approach. We profiled and
validated miRNA and mRNA expression in a unique series of
human colorectal metastasis tissues together with their
matched primary tumors and corresponding normal tissues. We
identified an exclusive miRNA signature that is
differentially expressed in metastases. Three of these
miRNAs were identified as key drivers of an EMT-regulating
network acting though a number of novel targets. These
targets include SIAH1, SETD2, ZEB2, and especially FOXN3,
which we demonstrated for the first time as a direct
transcriptional suppressor of N-cadherin. The modulation of
N-cadherin expression had significant impact on migration,
invasion, and metastasis in two different in vivo models.
The significant deregulation of the miRNAs defining the
network was confirmed in an independent patient set as well
as in a database of diverse malignancies derived from more
than 6,000 patients. Our data define a novel
metastasis-orchestrating network based on systematic
hypothesis generation from metastasis tissues.},
keywords = {Antigens, CD (NLM Chemicals) / CDH2 protein, human (NLM
Chemicals) / Cadherins (NLM Chemicals) / Cell Cycle Proteins
(NLM Chemicals) / FOXN3 protein, human (NLM Chemicals) /
Homeodomain Proteins (NLM Chemicals) / MIRN135 microRNA,
human (NLM Chemicals) / MIRN210 microRNA, human (NLM
Chemicals) / MIRN218 microRNA, human (NLM Chemicals) /
MicroRNAs (NLM Chemicals) / Nuclear Proteins (NLM Chemicals)
/ Repressor Proteins (NLM Chemicals) / ZEB2 protein, human
(NLM Chemicals) / Histone-Lysine N-Methyltransferase (NLM
Chemicals) / Set2 protein, human (NLM Chemicals) /
Ubiquitin-Protein Ligases (NLM Chemicals) / seven in
absentia proteins (NLM Chemicals)},
cin = {L201 / G360 / C060 / G200},
ddc = {610},
cid = {I:(DE-He78)L201-20160331 / I:(DE-He78)G360-20160331 /
I:(DE-He78)C060-20160331 / I:(DE-He78)G200-20160331},
pnm = {317 - Translational cancer research (POF3-317)},
pid = {G:(DE-HGF)POF3-317},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:26069251},
doi = {10.1158/0008-5472.CAN-15-0997},
url = {https://inrepo02.dkfz.de/record/127177},
}